First molecular characterization of Echinococcus granulosus (sensu stricto) genotype 1 among cattle in Sudan

被引：16
作者：
Ahmed, Mohamed E. [1 ]
Salim, Bashir [2 ]
Grobusch, Martin P. [3 ]
Aradaib, Imadeldin E. [1 ,4 ]
机构：
[1] Zamzam Univ Coll, EBH Res Ctr, Khartoum, Sudan
[2] Univ Khartoum, Fac Vet Med, Dept Parasitol, Khartoum, Sudan
[3] Univ Amsterdam, Amsterdam Med Ctr, Div Internal Med, Dept Infect Dis,Ctr Trop Med & Travel Med, Amsterdam, Netherlands
[4] Univ Khartoum, Fac Vet Med, Dept Clin Med, MBL, POB 32, Khartoum, Sudan
来源：
BMC VETERINARY RESEARCH | 2018年 / 14卷
关键词：
CYSTIC ECHINOCOCCOSIS; PHYLOGENY; STRAINS; HUMANS;
D O I：
10.1186/s12917-018-1348-9
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Background: Echinococcus granulosus sensu lato (s. l.) is the causative agent of cystic echinococcosis (CE), which is a cosmopolitan zoonotic parasitic disease infecting humans and a wide range of mammalian species including cattle. Currently, little information is available on the genetic diversity of Echinococcus species among livestock in Sudan. In the present study, fifty (n = 50) hydatid cysts were collected from cattle carcasses (one cyst sample per animal) at Al-kadarou slaughterhouse, Khartoum North, Sudan. DNA was extracted from protoscolices and the germinal layer of each cyst and subsequently amplified by PCR targeting the mitochondrial NADH dehydrogenase subunit 1 (NADH-1) gene. The amplified PCR products were purified and subjected to direct sequencing for subsequent construction of phylogenetic tree and net work analysis. Results: The phylogenetic tree revealed the presence of Echinococcus canadenesis genotype 6 (G6) in 44 cysts (88. 0%), Echinococcus ortleppi genotype 5 (G5) in 4 cysts (8.0%) and Echinococcus granulosus sensu stricto (s. s) genotype 1 (G1) in 2 cysts (4.0%). The phylogenetic network analysis revealed genetic variation among the different haplotypes/genotypes. This report has provided, for the first time, an insight of the role of cattle in the transmission of the zoonotic G1 echinococosis. Conclusions: The results of the study illustrate that Sudanese breeds of cattle may play an important role in the transmission dynamics and the epidemiology of cystic echinococcosis in Sudan. This study reports the first molecular identification of E. granulosus s. s. in cattle in Central Sudan.
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