Macroporous Resin Column Simulated Moving Bed Chromatography Adsorption for Separation of Albiflorin and Paeoniflorin

D101 macroporous resin column simulated moving bed separation process of albiflorin and paeoniflorin isomers was applicated in the paper. Determine the simulated moving bed separation conditions of albiflorin and paeoniflorin, separation column for the D101 macroporous resin column (ID. 26 mm X 400 mm), simulated moving bed chromatographic operation mode :1-4-3, sample concentration: 250 mg/mL, sampling flow rate: = 2 mL/min, elution rate: = 20 mL/min, extraction flow rate: = 35 mL/min, switching time: = 25 min, elution and extract:methanol: water (v/v) = 20 : 80. Calculated according to the standard curve, mass concentration of paeoniflorin in product was 90 %, the yield was 78 %; albiflorin was 91 %, the yield was 75 %. Under these conditions, the system can be stable operation. The relevant factors that impact of simulated moving bed separation was also discussed in the paper.