Galectin 3-β-galactobiose interactions

被引：16
作者：
Gunning, A. P. [1 ]
Pin, C. [1 ]
Morris, V. J. [1 ]
机构：
[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England
来源：
CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Modified pectin; Gal3; Anti-cancer action; beta-Galactobiose; Force spectroscopy; Atomic force microscopy; ATOMIC-FORCE MICROSCOPY; RHAMNOGALACTURONAN-I; AFFINITY SORBENT; PECTINS; RECOGNITION; PLANT; DISSOCIATION; COMPLEXES; STRENGTH; KINETICS;
D O I：
10.1016/j.carbpol.2012.08.104
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Force spectroscopy has been used to investigate the interaction between the disaccharide beta-galactobiose and the pro-metastatic regulatory protein galectin-3 (Gal3). The studies revealed specific interactions characterised by an off-rate dissociation constant k(off) = 0.33 s(-1) and interaction distance x=0.2 nm at zero applied force. These data suggest a lifetime for the interaction of 3.0 s. The results are consistent with the hypothesis that oral consumption of modified citrus pectin controls cancer metastasis by inhibiting the role of Gal3. The modification is considered to facilitate binding of pectin-derived galactan sidechains to Gal3 and inhibition of the roles of Gal3 as a pro-metastatic regulatory protein. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：529 / 533
页数：5
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