THE EFFECT OF BASIC FIBROBLAST GROWTH FACTOR (BFGF) ON REPAIRING INTESTINAL MUCOSA IN ACUTE COLITIS RAT

Objective: Inflammatory bowel disease (IBD) is a chronic and nonspecific inflammatory disease that persist for a long time and decrease the quality of life in patients. In this study, we explored the repair effect of basic fibroblast growth factor (bFGF) on intestinal mucosa in acute colitis rat. Methods: We established the acute colitis model by TNBS administration in rats. Acute colitis model rats were treated by bFGF with different doses, 60 mu g/kg (Low), 80 mu g/kg (Medium), 100 mu g/kg (High), respectively. Animal weight, stool, food and drink, and activity were observed. Immunohistochemistry (IHC) method was used to detect the expression of Claudin-1, Occludin, Zonula occludens-1(ZO-1),TGF-beta 1 in intestinal mucosa. The histopathology changes were assessed by HE staining. Ultrastructure of intestinal mucosa were assessed by transmission electron microscope. Results: bFGF treatment increased the animal weight (p<0.05). The IHC result showed that bFGF obviously increased the expression levels of Claudin-1, Occludin and ZO-1 while attenuated the expression levels of TGF-beta 1 in intestinal mucosa (p<0.05). In addition, bFGF ameliorated the histological damages, the parameters of injury/necrosis, inflammatory cell infiltration, submucosal edema, and mucosal bleeding, the histological score were all changed by bFGF treatment. Also, microvillus arrangement and cell arrangement in intestinal epithelium were improved after bFGF administration. The results were presented a dose dependent manner. Conclusion: bFGF have a repairing effect on intestinal mucosa in acute colitis rat, and its mechanisms may be attributed to regulate the expression of transmembrane proteins and anti-inflammatory cytokine.