Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system

被引：61

作者：

Crane, Matthew M. ^{[1
]}

Chung, Kwanghun ^{[2
]}

Lu, Hang ^{[1
,2
]}

机构：

[1] Georgia Inst Technol, Interdisciplinary Program Bioengn, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA USA

来源：

LAB ON A CHIP | 2009年 / 9卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

CAENORHABDITIS-ELEGANS; INTERFERENCE; RNA;

D O I：

10.1039/b813730g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Most visual screens based on fluorescent markers are currently limited in throughput and accuracy. Here we present the first genetic screen of a mutagenized population of C. elegans in a microfluidic device. Animal handling streamlined by a microfluidic device and intuitive control software enabled the identification of novel mutants and a large screening speed.

引用

页码：38 / 40

页数：3

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