The effects of cell recycling on the production of 1,3-propanediol by Klebsiella pneumoniae

被引：13

作者：

Avci, Fatma Gizem ^{[1
]}

Huccetogullari, Damla ^{[1
]}

Azbar, Nuri ^{[1
]}

机构：

[1] Ege Univ, Dept Bioengn, Fac Engn, TR-35100 Izmir, Turkey

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2014年 / 37卷 / 03期

关键词：

1,3-propanediol; Klebsiella pneumoniae; Cell recycling; By-product inhibition; MICROBIAL-PRODUCTION; MEMBRANE BIOREACTOR; CONTINUOUS-CULTURE; LACTIC-ACID; CLOSTRIDIUM-ACETOBUTYLICUM; GLYCEROL BIOCONVERSION; CITROBACTER-FREUNDII; AEROBIC CONDITIONS; PROPIONIC-ACID; WHEY PERMEATE;

D O I：

10.1007/s00449-013-1018-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of both biomass age and cell recycling on the 1,3-propanediol (1,3-PDO) production by Klebsiella pneumoniae were investigated in a membrane-supported bioreactor using hollow-fiber ultrafiltration membrane module in two separate experiments. It was determined that older cells have a negative effect on 1,3-PDO production. The concentrations of by-products, such as acetic acid and ethanol, increased in cultures with older cells, whereas the concentrations of succinic acid, lactic acid and 2,3-butanediol decreased. The effect of cell recycling was comparatively studied at a cell recycling ratio of 100 %. The results showed that cell recycling had also negative effects on 1,3-PDO fermentation. It was hypothesized that both cell recycling and biomass age caused metabolic shifts to undesired by-products which then inhibited the 1,3-PDO production. On the other hand, the use of hollow-fiber ultrafiltration membrane module was found to be very effective in terms of removal of cells from the fermentation broth.

引用

页码：513 / 519

页数：7

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