The effects of cell recycling on the production of 1,3-propanediol by Klebsiella pneumoniae

被引：13

作者：

Avci, Fatma Gizem ^{[1
]}

Huccetogullari, Damla ^{[1
]}

Azbar, Nuri ^{[1
]}

机构：

[1] Ege Univ, Dept Bioengn, Fac Engn, TR-35100 Izmir, Turkey

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2014年 / 37卷 / 03期

关键词：

1,3-propanediol; Klebsiella pneumoniae; Cell recycling; By-product inhibition; MICROBIAL-PRODUCTION; MEMBRANE BIOREACTOR; CONTINUOUS-CULTURE; LACTIC-ACID; CLOSTRIDIUM-ACETOBUTYLICUM; GLYCEROL BIOCONVERSION; CITROBACTER-FREUNDII; AEROBIC CONDITIONS; PROPIONIC-ACID; WHEY PERMEATE;

D O I：

10.1007/s00449-013-1018-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of both biomass age and cell recycling on the 1,3-propanediol (1,3-PDO) production by Klebsiella pneumoniae were investigated in a membrane-supported bioreactor using hollow-fiber ultrafiltration membrane module in two separate experiments. It was determined that older cells have a negative effect on 1,3-PDO production. The concentrations of by-products, such as acetic acid and ethanol, increased in cultures with older cells, whereas the concentrations of succinic acid, lactic acid and 2,3-butanediol decreased. The effect of cell recycling was comparatively studied at a cell recycling ratio of 100 %. The results showed that cell recycling had also negative effects on 1,3-PDO fermentation. It was hypothesized that both cell recycling and biomass age caused metabolic shifts to undesired by-products which then inhibited the 1,3-PDO production. On the other hand, the use of hollow-fiber ultrafiltration membrane module was found to be very effective in terms of removal of cells from the fermentation broth.

引用

页码：513 / 519

页数：7

共 47 条

[11] Development of a biofilm technology for the production of 1,3-propanediol (1,3-PDO) from crude glycerol [J].

Casali, Silvia ;

Gungormusler, Mine ;

Bertin, Lorenzo ;

Fava, Fabio ;

Azbar, Nuri .

BIOCHEMICAL ENGINEERING JOURNAL, 2012, 64 :84-90

[12] EXTRACTIVE ETHANOL-PRODUCTION IN A MEMBRANE CELL RECYCLE BIOREACTOR [J].

CHANG, HN ;

YANG, JW ;

PARK, YS ;

KIM, DJ ;

HAN, KC .

JOURNAL OF BIOTECHNOLOGY, 1992, 24 (03) :329-343

[13] Multiple growth inhibition of Klebsiella pneumoniae in 1,3-propanediol fermentation [J].

Cheng, KK ;

Liu, HJ ;

Liu, DH .

BIOTECHNOLOGY LETTERS, 2005, 27 (01) :19-22

[14] PRODUCTION OF PROPIONIC-ACID FROM WHEY PERMEATE BY SEQUENTIAL FERMENTATION, ULTRAFILTRATION, AND CELL RECYCLING [J].

COLOMBAN, A ;

ROGER, L ;

BOYAVAL, P .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (09) :1091-1098

[15] Glycerol: A promising and abundant carbon source for industrial microbiology [J].

da Silva, Gervasio Paulo ;

Mack, Matthias ;

Contiero, Jonas .

BIOTECHNOLOGY ADVANCES, 2009, 27 (01) :30-39

[16] 3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae [J].

Hao, Jian ;

Lin, Rihui ;

Zheng, Zongming ;

Sun, Yan ;

Liu, Dehua .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2008, 35 (12) :1615-1624

[17] Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions [J].

Hao, Jian ;

Lin, Rihui ;

Zheng, Zongming ;

Liu, Hongjuan ;

Liu, Dehua .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24 (09) :1731-1740

[18]

HOMANN T, 1990, APPL MICROBIOL BIOT, V33, P121

[19] Production of 1,3-propanediol by Klebsiella pneumoniae [J].

Huang, H ;

Gong, CS ;

Tsao, GT .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2002, 98 (1-9) :687-698

[20] Synthetic methods for the preparation of 1,3-propanediol [J].

Kraus, George A. .

CLEAN-SOIL AIR WATER, 2008, 36 (08) :648-651

← 1 2 3 4 5 →