Glutathione-s-transferase based electrochemical biosensor for the detection of captan

被引：16

作者：

Singh, Ravindra P. ^{[2
]}

Kim, Young Jun ^{[2
]}

Oh, Byung-Keun ^{[1
,2
]}

Choi, Jeong-Woo ^{[1
,2
]}

机构：

[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea

[2] Sogang Univ, Interdisciplinary Program Integrated Biotechnol, Seoul 121742, South Korea

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2009年 / 11卷 / 01期

关键词：

Glutathione-S-transferase; Captan; Bioelectrodes; Cyclic voltammetry; AFM and STM; OPTICAL BIOSENSOR;

D O I：

10.1016/j.elecom.2008.11.003

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The proposed electrochemical biosensor based on the inhibition of glutathione-s-transferase (GST) onto SAM modified gold due to captan was developed and determined by CV technique. The bioelectrode exhibited improved fast response time (12 s) with the detection lit-nits 0.25-16 ppm, percentage inhibition >72% and high sensitivity 4.5 uA/ppm at standard optimal conditions. The recovery experiment results were found between 77% and 144% from spiked water sources. The bioelectrode was regenerated by DTT and reusable. The bioelectrodes were characterized by UV-visible, CV, AFM and STM. Thus, the proposed electrochemical biosensor is not only detected captan but also its metabolite and promising for real-time analysis of small molecules of environmental interest. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：181 / 185

页数：5

共 12 条

[1]

[Anonymous], EPA630P03001F

[2] Optical biosensor for simultaneous detection of captan and organophosphorus compounds [J].

Choi, JW ;

Kim, YK ;

Oh, BK ;

Song, SY ;

Lee, WH .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (5-6) :591-597

[3] Optical biosensor consisting of glutathione-S-transferase for detection of captan [J].

Choi, JW ;

Kim, YK ;

Song, SY ;

Lee, IH ;

Lee, WH .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (12) :1461-1466

[4]

DEBAUN JR, 1974, XENOBIOTICA, V4, P101, DOI 10.3109/00498257409049350

[5] Immobilisation of glucose oxidase within metallic nanotubes arrays for application to enzyme biosensors [J].

Delvaux, M ;

Demoustier-Champagne, S .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (07) :943-951

[6] Interaction of glutathione transferase P1-1 with captan and captafol [J].

DiIlio, C ;

Sacchetta, P ;

Angelucci, S ;

Bucciarelli, T ;

Pennelli, A ;

Mazzetti, AP ;

LoBello, M ;

Aceto, A .

BIOCHEMICAL PHARMACOLOGY, 1996, 52 (01) :43-48

[7] Comparison of the efficiencies of different types of adsorbents at trapping currently used pesticides in the gaseous phase using the technique of high-volume sampling [J].

Dobson, Rowan ;

Scheyer, Anne ;

Rizet, Anne Laure ;

Mirabel, Philippe ;

Millet, Maurice .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 386 (06) :1781-1789

[8]

HABIG WH, 1974, J BIOL CHEM, V249, P7130

[9] Patterning of conducting polymer nanowires on gold/platinum electrodes [J].

Kemp, Neil T. ;

Cochrane, Jackw ;

Newbury, Richard .

NANOTECHNOLOGY, 2007, 18 (14)

[10] CAPTAN METABOLISM IN HUMANS YIELDS 2 BIOMARKERS, TETRAHYDROPHTHALIMIDE (THPI) AND THIAZOLIDINE-2-THIONE-4-CARBOXYLIC ACID (TTCA) IN URINE [J].

KRIEGER, RI ;

THONGSINTHUSAK, T .

DRUG AND CHEMICAL TOXICOLOGY, 1993, 16 (02) :207-225

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