Effect of phytase on growth performance, phytate degradation and gene expression of myo-inositol transporters in the small intestine, liver and kidney of 21 day old broilers

被引:53
作者
Walk, C. L. [1 ]
Bedford, M. R. [1 ]
Olukosi, O. A. [2 ]
机构
[1] AB Vista, Marlborough, Wilts, England
[2] Scotlands Rural Coll, Monogastr Sci Res Ctr, Edinburgh EH9 3JG, Midlothian, Scotland
关键词
broiler; gene expression; myo-inositol; phytase; phytate; MICROBIAL PHYTASE; INOSITOL; SUPERDOSES; MEMBRANE; POULTRY; ESTERS; DIETS; SMIT2; PH;
D O I
10.3382/ps/pex392
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
An experiment was conducted to evaluate phytase supplementation on growth, phytate degradation, and the gene expression of myo-inositol transporters in 21-day old broilers. Ross 308, male broilers (n = 240) were assigned to one of four diets, with 10 pens/diet and six birds/pen from day one to 21. The diets consisted of a negative control (NC) formulated to meet or exceed Ross 308 nutrient requirements, with the exception of calcium (Ca) and available P (avP), which were reduced by 0.16 and 0.15%, respectively. The NC diet was supplemented with 0, 500, 1,500, or 4,500 units/kg of phytase (FTU) to create four experimental diets. On day 21, all birds per pen were euthanized to obtain digesta and tissue samples for phytate degradation and gene expression. Data were analyzed as an analysis of variance using the fit model platform in JMP v 13.0. The model included phytase and significant means were separated using orthogonal linear and quadratic contrasts. Phytase supplementation increased gain (linear, P < 0.05). Phytate (iP6; quadratic, P < 0.05), phytate ester (iP5, iP4, iP3; quadratic, P < 0.05), and inositol (linear, P < 0.05) concentration in the gizzard was influenced by phytase supplementation. Phytate concentration decreased (linear, P < 0.05), iP5 or iP4 concentration increased and then decreased (quadratic, P < 0.05), and inositol concentration increased (quadratic, P < 0.05) in the ileal digesta as phytase supplementation increased in the diet. There was a tendency for the gene expression of the H+-dependent myo-inositol transporter, HMIT, to increase (linear, P < 0.05) in the ileum as phytase dose increased. Gene expression of the sodium-dependent myo-inositol transporter, SMIT2, increased in the jejunum (quadratic, P < 0.05) as phytase dose increased. Intestinal alkaline phosphatase expression increased (linear, P < 0.05) in the ileum as phytase supplementation increased in the diet. The influence of phytase on phytate, phytate esters, and inositol may influence intestinal alkaline phosphatase activity and the gene expression of myo-inositol transporters in the small intestine.
引用
收藏
页码:1155 / 1162
页数:8
相关论文
共 30 条
[1]  
AOAC International, 2006, OFFICIAL METHODS ANA, V18th
[2]   SMIT2 mediates all myo-inositol uptake in apical membranes of rat small intestine [J].
Aouameur, Rym ;
Da Cal, Sandra ;
Bissonnette, Pierre ;
Coady, Michael J. ;
Lapointe, Jean-Yves .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2007, 293 (06) :G1300-G1307
[3]  
Bedford MR, 2016, PHYTATE DESTRUCTION: CONSEQUENCES FOR PRECISION ANIMAL NUTRITION, P45, DOI 10.3920/978-90-8686-836-0_3
[4]   Hydrolysis of phytate to its lower esters can influence the growth performance and nutrient utilization of broilers with regular or super doses of phytase [J].
Beeson, L. A. ;
Walk, C. L. ;
Bedford, M. R. ;
Olukosi, O. A. .
POULTRY SCIENCE, 2017, 96 (07) :2243-2253
[5]   High-performance ion chromatography method for separation and quantification of inositol phosphates in diets and digesta [J].
Blaabjerg, K. ;
Hansen-Moller, J. ;
Poulsen, H. D. .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (3-4) :347-354
[6]   Supplementation of corn-soy-based diets with an Eschericia coli-derived phytase:: Effects on broiler chick performance and the digestibility of amino acids and metabolizability of minerals and energy [J].
Cowieson, A. J. ;
Acamovic, T. ;
Bedford, M. R. .
POULTRY SCIENCE, 2006, 85 (08) :1389-1397
[7]   Possible involvement of myo-inositol in the physiological response of broilers to high doses of microbial phytase [J].
Cowieson, A. J. ;
Aureli, R. ;
Guggenbuhl, P. ;
Fru-Nji, F. .
ANIMAL PRODUCTION SCIENCE, 2015, 55 (06) :710-719
[8]   The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets [J].
Cowieson, A. J. ;
Ptak, A. ;
Mackowiak, P. ;
Sassek, M. ;
Pruszynska-Oszmalek, E. ;
Zyla, K. ;
Swiatkiewicz, S. ;
Kaczmarek, S. ;
Jozefiak, D. .
POULTRY SCIENCE, 2013, 92 (08) :2124-2134
[9]   Super-dosing effects of phytase in poultry and other monogastrics [J].
Cowieson, A. J. ;
Wilcock, P. ;
Bedford, M. R. .
WORLDS POULTRY SCIENCE JOURNAL, 2011, 67 (02) :225-235
[10]   Potential role and therapeutic interests of myo-inositol in metabolic diseases [J].
Croze, Marine L. ;
Soulage, Christophe O. .
BIOCHIMIE, 2013, 95 (10) :1811-1827