Cytochromes P450 Preferentially Expressed in Antennae of the Mountain Pine Beetle

被引：19

作者：

Chiu, Christine C. ^{[1
,2
]}

Keeling, Christopher I. ^{[1
,3
]}

Bohlmann, Joerg ^{[1
,2
,4
]}

机构：

[1] Univ British Columbia, Michael Smith Labs, 2185 East Mall, Vancouver, BC V6T 1Z4, Canada

[2] Univ British Columbia, Dept Bot, 6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada

[3] Nat Resources Canada, Laurentian Forestry Ctr, Canadian Forest Serv, 1055 Rue PEPS,Stn St Foy,POB 1038, Quebec City, PQ G1V 4C7, Canada

[4] Univ British Columbia, Dept Forest & Conservat Sci, 2441 Main Mall, Vancouver, BC V6T 1Z4, Canada

来源：

JOURNAL OF CHEMICAL ECOLOGY | 2019年 / 45卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Cytochromes P450; Bark beetles; Olfaction; Detoxification; Pheromone biosynthesis; Mountain pine beetle; DENDROCTONUS-PONDEROSAE HOPKINS; BARK BEETLES; CURCULIONIDAE SCOLYTINAE; PHEROMONE PRODUCTION; CONIFER BARK; IPS-PINI; COLEOPTERA; HOST; DEFENSE; GENES;

D O I：

10.1007/s10886-018-0999-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mountain pine beetle (MPB, Dendroctonus ponderosae) is a forest insect pest endemic to western North America. During dispersal and host colonization, MPB identify suitable host trees by olfaction of monoterpene volatiles, contend with host terpene defenses, and communicate with conspecifics using terpenoid and other pheromones. Cytochromes P450 (P450s) have been proposed to function in MPB olfaction, terpene detoxification, and pheromone biosynthesis. Here, we identified P450s that were abundant in the antennae transcriptome. Analysis of transcript levels across different life stages and tissues in females and males showed additional expression of most of these P450s in the midgut or fat body. These expression profiles suggest specific or overlapping functions in olfaction, detoxification, and pheromone biosynthesis.

引用

页码：178 / 186

页数：9

共 51 条

[1] Mountain Pine Beetles Colonizing Historical and Naive Host Trees Are Associated with a Bacterial Community Highly Enriched in Genes Contributing to Terpene Metabolism [J].

Adams, Aaron S. ;

Aylward, Frank O. ;

Adams, Sandye M. ;

Erbilgin, Nadir ;

Aukema, Brian H. ;

Currie, Cameron R. ;

Suen, Garret ;

Raffa, Kenneth F. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (11) :3468-3475

[2]

[Anonymous], 2009, USEFUL PROCEDURE ISO, DOI [10.1038/nprot.2009.163, DOI 10.1038/NPROT.2009.163]

[3]

[Anonymous], 2017, R LANG ENV STAT COMP

[4] Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fat bodies [J].

Aw, Tidiane ;

Schlauch, Karen ;

Keeling, Christopher I. ;

Young, Sharon ;

Bearfield, Jeremy C. ;

Blomquist, Gary J. ;

Tittiger, Claus .

BMC GENOMICS, 2010, 11

[5] Properties of sufficiency and statistical tests [J].

Bartlett, MS .

PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1937, 160 (A901) :0268-0282

[6]

Benjamini Y, 2001, ANN STAT, V29, P1165

[7] Determining the instar of mountain pine beetle (Coleoptera: Curculionidae) larvae by the width of their head capsules [J].

Bleiker, K. P. ;

Regniere, J. .

CANADIAN ENTOMOLOGIST, 2015, 147 (05) :635-640

[8] Bacteria Associated with a Tree-Killing Insect Reduce Concentrations of Plant Defense Compounds [J].

Boone, Celia K. ;

Keefover-Ring, Ken ;

Mapes, Abigail C. ;

Adams, Aaron S. ;

Bohlmann, Joerg ;

Raffa, Kenneth F. .

JOURNAL OF CHEMICAL ECOLOGY, 2013, 39 (07) :1003-1006

[9]

Boone CK, 2011, CAN J FOREST RES, V41, P1174, DOI [10.1139/X11-041, 10.1139/x11-041]

[10]

Borden JH, 2008, J ECON ENTOMOL, V101, P1266, DOI 10.1603/0022-0493(2008)101[1266:SBOMFA]2.0.CO

← 1 2 3 4 5 6 →