Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry

被引：168

作者：

Zhou, Wangting ^{[1
]}

Yan, Yamei ^{[2
]}

Mi, Jia ^{[2
]}

Zhang, Hongcheng ^{[3
]}

Lu, Lu ^{[2
]}

Luo, Qing ^{[2
]}

Li, Xiaoying ^{[2
]}

Zeng, Xiaoxiong ^{[1
]}

Cao, Youlong ^{[2
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

[2] Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Peoples R China

[3] Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 04期

关键词：

bee collected pollen from Chinese wolfberry; polysaccharides; simulated digestion; fermentation; short-chain fatty acids; gut microbiota; MULBERRY FRUIT POLYSACCHARIDES; CHAIN FATTY-ACIDS; INTESTINAL MICROBIOTA; ANTIOXIDANT ACTIVITY; HEALTH; SALIVA; METABOLISM; BUTYRATE; IMPACT; COLON;

D O I：

10.1021/acs.jafc.7b05546

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study. It was found that WBPPS mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively. WBPPS was not affected by human saliva. The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded. Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XIVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium.

引用

页码：898 / 907

页数：10

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