A New Approach to Assess Gold Nanoparticle Uptake by Mammalian Cells: Combining Optical Dark-Field and Transmission Electron Microscopy

被引：58

作者：

Rosman, Christina ^{[1
]}

Pierrat, Sebastien ^{[2
]}

Henkel, Andreas ^{[1
]}

Tarantola, Marco ^{[3
]}

Schneider, David ^{[4
]}

Sunnick, Eva ^{[4
]}

Janshoff, Andreas ^{[4
]}

Soennichsen, Carsten ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Phys Chem, D-55128 Mainz, Germany

[2] Fraunhofer Inst Microelect Circuits & Syst IMS, D-47057 Duisburg, Germany

[3] Max Planck Inst Dynam & Self Org, Lab Fluid Dynam Pattern Format & Biocomplex LFPB, D-37077 Gottingen, Germany

[4] Univ Gottingen, Inst Phys Chem, D-37077 Gottingen, Germany

来源：

SMALL | 2012年 / 8卷 / 23期

关键词：

cellular uptake; gold; nanoparticles; microscopy; quantification; CELLULAR UPTAKE; SURFACE-CHEMISTRY; TOXICITY; CYTOTOXICITY; NANORODS; SIZE; INTERNALIZATION; PATHWAY; SHAPE;

D O I：

10.1002/smll.201200853

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Toxicological effects of nanoparticles are associated with their internalization into cells. Hence, there is a strong need for techniques revealing the interaction between particles and cells as well as quantifying the uptake at the same time. For that reason, herein optical dark-field microscopy is used in conjunction with transmission electron microscopy to investigate the uptake of gold nanoparticles into epithelial cells with respect to shape, stabilizing agent, and surface charge. The number of internalized particles is strongly dependent on the stabilizing agent, but not on the particle shape. A test of metabolic activity shows no direct correlation with the number of internalized particles. Therefore, particle properties besides coating and shape are suspected to contribute to the observed toxicity.

引用

页码：3683 / 3690

页数：8

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