Removal of water-insoluble Sudan dyes by Shewanella oneidensis MR-1

被引：25

作者：

Ji, Qiuyan ^{[1
]}

Liu, Guangfei ^{[1
]}

Zhou, Jiti ^{[1
]}

Wang, Jing ^{[1
]}

Jin, Ruofei ^{[1
]}

Lv, Hong ^{[1
]}

机构：

[1] Dalian Univ Technol, Sch Environm Sci & Technol, Minist Educ, Key Lab Ind Ecol & Environm Engn, Dalian 116024, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 114卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Shewanella oneidensis MR-1; Water-insoluble dye; Sudan dye; Reduction; AZO DYES; DECOLORATIONIS S12; ELECTRON-ACCEPTOR; PARA RED; REDUCTION; DECOLORIZATION; DEGRADATION; RESPIRATION; BACTERIA; CELLS;

D O I：

10.1016/j.biortech.2012.03.014

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Decolorization of water-insoluble Sudan dyes was studied with Shewanella oneidensis MR-1, which removed 66.8%, 43.4%, 56.0% and 33.7% Sudan I-IV in 104 h, respectively and reduced Sudan I to aniline and 1-amino-2-naphthol. Lactate was identified as the most efficient electron donor for Sudan I reduction. Improved reduction performance was obtained in the presence of higher lactate or biomass concentration. The correlation between specific reduction rate and initial Sudan I concentration could be described with Michaelis-Menten kinetics (V-max = 1.8 mg Sudan I mg cell(-1) h(-1) and K-m = 5.3 mg l(-1)). The addition of anthraquinone-2-sulfonate stimulated the reduction significantly whereas the presence of 2-hydroxy-1,4-naphthoquinone had little enhancing effect. The main azoreductase activity was found with membrane-bound proteins of MR-1 and no reduction occurred when Sudan I was incubated with cell extracts. These data indicated for the first time that Shewanella could reduce solid-phase Sudan dye particles. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：144 / 148

页数：5

共 34 条

[31] Anaerobic metabolism of 1-amino-2-naphthol-based azo dyes (Sudan dyes) by human intestinal microflora [J].