Construction of castor functional markers fingerprint and analysis of genetic diversity

In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources, fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers (FMs) associated with fatty acid metabolism-related genes. A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103-0.695. Shannon's information index (I), observed heterozygosity (Ho) and expected heterozygosity (He) were 0.699, 0.188 and 0.436 respectively. The clustering results indicated that the castor germplasm could be divided into two groups with the genetic similarity coefficient of 0.59. The genetic similarity of 12 regions ranged from 0.518 to 0.917 and the genetic distance was between 0.087 and 0.658. A total of 5 pairs of core primers were screened to construct a digital fingerprint of different castor germplasm resources, which could distinguish all 52 germplasms. This study provides a scientific basis for screening high-quality castor germplasm resources and broadening the genetic basis of castor breeding at the molecular level.