HPLC analysis of geometrical isomers of lutein epoxide isolated from dandelion (Taraxacum officinale F.!Weber ex Wiggers)

被引：48

作者：

Melendez-Martinez, Antonio J.

Britton, George

Vicario, Isabel M.

Heredia, Francisco J.

机构：

[1] Univ Sevilla, Fac Pharm, Dept Nutr & Food Sci, Lab Food Colour & Qual, E-41012 Seville, Spain

[2] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England

来源：

PHYTOCHEMISTRY | 2006年 / 67卷 / 08期

关键词：

Taraxacum officinale F. Weber ex Wiggers; Asteraceae; dandelion; carotenoids; geometrical isomers; lutein epoxide; taraxanthin;

D O I：

10.1016/j.phytochem.2006.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lutein epoxide has been isolated from petals of dandelion (Taraxacum officinale F. Weber ex Wiggers) by thin-layer chromatography (TLC) on silica to be used for the accurate identification of this carotenoid in other sources. The extract was analyzed by high-performance liquid chromatography (HPLC) using a C-30 column, as a result of which six geometrical isomers were separated. The identification of these isomers was performed on the basis of their UV/vis spectroscopic features in the mobile phase. In quantitative terms, it was observed that all-E-lutein epoxide was the major carotenoid and that there were also high amounts of the (9Z)- and (9'Z)-isomers, although the latter may be an artifact. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：771 / 777

页数：7

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