LC-ESI-MS quali-quantitative determination of phenolic constituents in different parts of wild and cultivated Astragalus gombiformis

被引：36

作者：

Montoro, Paola ^{[1
]}

Teyeb, Hassen ^{[2
]}

Masullo, Milena ^{[1
]}

Mari, Angela ^{[1
]}

Douki, Wahiba ^{[2
]}

Piacente, Sonia ^{[1
]}

机构：

[1] Univ Salerno, Dipartimento Sci Farmaceut & Biomed, I-84084 Fisciano, SA, Italy

[2] Univ Hosp Fattouma Bourguiba, Toxicol & Biochem Lab, Monastir 5000, Tunisia

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2013年 / 72卷

关键词：

Astragalus gombiforrnis; LC-ESI-MS profiling; Flavonol glycosides acylated with 3-hydroxy-3-methylglutaric acid; Quantitative analyses; Principal Component Analysis; CYCLOARTANE GLYCOSIDES; FLAVONOL GLYCOSIDES; RADIX-ASTRAGALI; TRITERPENE GLYCOSIDES; SAPONINS; PRODUCTS; EXTRACT; ACID;

D O I：

10.1016/j.jpba.2012.09.014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

High-performance liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) profiling of the MeOH extract of Astragalus gombiformis Pomel (Fabaceae) aerial parts guided the isolation of seven phenolic compounds among which 7-methylquercetin 3-O-alpha-L-rhamnopyranosyl-(1 -> 2)-beta-D-galactopyranoside (2) and 7-methylquercetin 3-O-alpha-L-rhamnopyranosyl-(1 -> 2)-[6-O-(3-hydroxy-3-methylglutaryl)-beta-D-galactopyranoside] (7) whose structures were elucidated by NMR and ESI-MS experiments. The radical scavenging activities of isolated compounds were investigated, by using the TEAC assay. Furthermore quantitative analyses were performed by LC-ESI-MS and applied to the comparative profiling of different parts (aerial parts, leaves and stems) of cultivated and wild A. gombiformis samples, confirming the interest of these compounds as markers of the species. Finally, a Principal Component Analysis was carried out in order to highlight the differences between different parts of cultivated and wild plants. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：89 / 98

页数：10

共 40 条

[31]

Savran T, 2012, REC NAT PROD, V6, P230

[32] Four new flavonol glycosides from the leaves of Astragalus caprinus [J].