Nitrogenase phylogeny and the molybdenum dependence of nitrogen fixation in Methanococcus maripaludis

被引:34
|
作者
Kessler, PS [1 ]
McLarnan, J [1 ]
Leigh, JA [1 ]
机构
[1] UNIV WASHINGTON,DEPT MICROBIOL,SEATTLE,WA 98195
关键词
D O I
10.1128/jb.179.2.541-543.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We studied the effects of molybdenum, vanadium, and tungsten on the diazotrophic growth of Methanococus maripaludis. Mo stimulated growth, with a maximal response at 4.0 mu M, while V had no effect at any concentration tested. W specifically inhibited diazotrophic growth in the presence of Mo. Coupling the results of our analysis and other known metal requirements with phylogenies derived from nifD and nifK genes revealed distinct clusters for Mo-, V-, and Fe-dinitrogenases and suggested that most methanogens also have molybdenum-type nitrogenases.
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页码:541 / 543
页数:3
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