Routine identification of Nocardia species by MALDI-TOF mass spectrometry

被引：39

作者：

Girard, Victoria ^{[1
]}

Mailler, Sandrine ^{[1
]}

Polsinelli, Sophie ^{[1
]}

Jacob, Delphine ^{[1
]}

Saccomani, Marie Christine ^{[1
]}

Celliere, Beatrice ^{[1
]}

Monnin, Valerie ^{[1
]}

van Belkum, Alex ^{[1
]}

Hagen, Ferry ^{[2
]}

Meis, Jacques F. ^{[2
,3
]}

Durand, Geraldine ^{[1
]}

机构：

[1] BioMerieux, R&D Microbiol, La Balme Les Grottes, France

[2] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands

[3] Radboud Univ Nijmegen, Dept Med Microbiol, Med Ctr, Nijmegen, Netherlands

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 87卷 / 01期

关键词：

MALDI TOF MS; Nocardia; Species identification; Routine diagnostic microbiology; DESORPTION IONIZATION-TIME; MYCOBACTERIUM; INFECTION;

D O I：

10.1016/j.diagmicrobio.2016.09.024

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0). (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：7 / 10

页数：4

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