Inhibitory effects of baicalin on ultraviolet B-induced photo-damage in keratinocyte cell line

被引：23

作者：

Min, Wei ^{[1
]}

Lin, Xiang-Fei ^{[1
]}

Miao, Xu ^{[1
]}

Wang, Bao-Tao ^{[2
]}

Yang, Zi-Liang ^{[2
]}

Luo, Dan ^{[1
]}

机构：

[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Dermatol, Nanjing 210029, Peoples R China

[2] Soochow Univ, Affiliated Hosp 1, Dept Dermatol, Suzhou 215006, Peoples R China

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2008年 / 36卷 / 04期

基金：

中国国家自然科学基金;

关键词：

baicalin; ultraviolet B; IL-6 and TNF-alpha; c-fos; p53/p21; PCNA; RPA;

D O I：

10.1142/S0192415X0800620X

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Baicalin, one kind of Chinese herbal medicine with anti-inflammatory and antioxidant property, has been commonly used as a clinical medicine. However, little has been known about the effects of Baicalin on ultraviolet (UV) induced photo-aging and photo-carcinogenesis. The photoproduct is critical to the initial event of UV-induced photo-carcinogenesis. The purpose of the present study was to investigate whether Baicalin, in immortalized human keratinocyte HaCaT cells, could inhibit ultraviolet-B (UVB) induced skin damage and its possible underlying mechanisms, such as inhibiting UVB-induced cytotoxicity and apoptosis, cyclobutane pyrimidine dimers (CPDs), down-regulating the expression of regulatory proteins which are related to cell apoptosis and DNA damage/repair. Our study revealed that Baicalin treatment could inhibit the UVB-induced cytotoxicity, apoptosis and CPD level. It also decreased the mRNA expression of apoptosis-regulatory genes (p53-p21 and c-fos), the protein levels of p53, proliferating cell nuclear antigen (PCNA) and repair protein A (RPA), and the secretion of cytokines [interleukin(IL)-6 and tumor necrosis factor (TNF-alpha)]. These results suggested that Baicalin may have an inhibitory effect on the UVB-induced photo-damage by blocking the relevant cytokine secretion and expression of p53-p21, c-fos, PCNA and RPA genes.

引用

页码：745 / 760

页数：16

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