Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids

被引：108

作者：

Preus, Soren ^{[2
]}

Wilhelmsson, L. Marcus ^{[1
]}

机构：

[1] Chalmers Univ Technol, Dept Chem & Biol Engn, S-41296 Gothenburg, Sweden

[2] Univ Copenhagen, Dept Chem, DK-2100 Copenhagen, Denmark

来源：

CHEMBIOCHEM | 2012年 / 13卷 / 14期

基金：

瑞典研究理事会;

关键词：

DNA structures; fluorescence spectroscopy; fluorescent probes; FRET; nucleic acids; RESONANCE ENERGY-TRANSFER; SINGLE-MOLECULE FRET; ALTERNATING-LASER EXCITATION; DOUBLE-STRANDED DNA; NANO-POSITIONING SYSTEM; PHOTOINDUCED ELECTRON-TRANSFER; FLUORESCENCE PROPERTIES; CONFORMATIONAL DYNAMICS; PHOTON DISTRIBUTION; STRUCTURAL HETEROGENEITIES;

D O I：

10.1002/cbic.201200400

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Forster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for on/off experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.

引用

页码：1990 / 2001

页数：12

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