Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models

被引：203

作者：

Delvecchio, Rodrigo ^{[1
]}

Higa, Luiza M. ^{[1
]}

Pezzuto, Paula ^{[1
]}

Valadao, Ana Luiza ^{[1
]}

Garcez, Patricia P. ^{[2
,3
]}

Monteiro, Fabio L. ^{[1
]}

Loiola, Erick C. ^{[3
]}

Dias, Andre A. ^{[4
]}

Silva, Fabio J. M. ^{[2
]}

Aliota, Matthew T. ^{[5
]}

Caine, Elizabeth A. ^{[5
]}

Osorio, Jorge E. ^{[5
]}

Bellio, Maria ^{[4
]}

O'Connor, David H. ^{[6
]}

Rehen, Stevens ^{[2
,3
]}

de Aguiar, Renato Santana ^{[1
]}

Savarino, Andrea ^{[7
]}

Campanati, Loraine ^{[2
]}

Tanuri, Amilcar ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biol, Dept Genet, BR-21941902 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Biomed Sci, BR-21941902 Rio De Janeiro, Brazil

[3] DOr Inst Res & Educ IDOR, BR-22281100 Rio De Janeiro, Brazil

[4] Univ Fed Rio de Janeiro, Dept Immunol, BR-21941902 Rio De Janeiro, Brazil

[5] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA

[6] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 USA

[7] Ist Super Sanita, Deptartment Infect Dis, 299 Viale Regina Elena, I-00161 Rome, Italy

来源：

VIRUSES-BASEL | 2016年 / 8卷 / 12期

关键词：

Zika virus; chloroquine; antiviral; microcephaly; neural stem cell; SEXUAL TRANSMISSION; MECHANISM; PREGNANCY; ENTRY; DRUG; REPLICATION; MICRONESIA; ORGANOIDS; EXPOSURE; MALARIA;

D O I：

10.3390/v8120322

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres.

引用

页数：15

共 56 条

[1] A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection [J].

Barrows, Nicholas J. ;

Campos, Rafael K. ;

Powell, Steven T. ;

Prasanth, K. Reddisiva ;

Schott-Lerner, Geraldine ;

Soto-Acosta, Ruben ;

Galarza-Munoz, Gaddiel ;

McGrath, Erica L. ;

Urrabaz-Garza, Rheanna ;

Gao, Junling ;

Wu, Ping ;

Menon, Ramkumar ;

Saade, George ;

Fernandez-Salas, Ildefonso ;

Rossi, Shannan L. ;

Vasilakis, Nikos ;

Routh, Andrew ;

Bradrick, Shelton S. ;

Garcia-Blanco, Mariano A. .

CELL HOST & MICROBE, 2016, 20 (02) :259-270

[2] Type III Interferons Produced by Human Placental Trophoblasts Confer Protection against Zika Virus Infection [J].

Bayer, Avraham ;

Lennemann, Nicholas J. ;

Ouyang, Yingshi ;

Bramley, John C. ;

Morosky, Stefanie ;

De Azeved Marques, Ernesto Torres, Jr. ;

Cherry, Sara ;

Sadovsky, Yoel ;

Coyne, Carolyn B. .

CELL HOST & MICROBE, 2016, 19 (05) :705-712

[3]

BEARCROFT W. G. C., 1956, TRANS ROY SOC TROP MED AND HYG, V50, P442, DOI 10.1016/0035-9203(56)90090-6

[4] Amodiaquine, an antimalarial drug, inhibits dengue virus type 2 replication and infectivity [J].

Boonyasuppayakorn, Siwaporn ;

Reichert, Erin D. ;

Manzano, Mark ;

Nagarajan, Kuppuswamy ;

Padmanabhan, Radhakrishnan .

ANTIVIRAL RESEARCH, 2014, 106 :125-134

[5] Chloroquine use improves dengue-related symptoms [J].

Borges, Marcos Carvalho ;

Castro, Luiza Antunes ;

Lopes da Fonseca, Benedito Antonio .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2013, 108 (05) :596-599

[6]

Brasil P., 2016, ENGL J MED, DOI [10.1056/NEJMoa160241226943629, DOI 10.1056/NEJMOA160241226943629]

[7]

Browning D., 2014, PHARM CHLOROQUINE HY, V35, P63

[8] Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study [J].

Calvet, Guilherme ;

Aguiar, Renato S. ;

Melo, Adriana S. O. ;

Sampaio, Simone A. ;

de Filippis, Ivano ;

Fabri, Allison ;

Araujo, Eliane S. M. ;

de Sequeira, Patricia C. ;

de Mendonca, Marcos C. L. ;

de Oliveira, Louisi ;

Tschoeke, Diogo A. ;

Schrago, Carlos G. ;

Thompson, Fabiano L. ;

Brasil, Patricia ;

dos Santos, Flavia B. ;

Nogueira, Rita M. R. ;

Tanurit, Amilcar ;

de Filippis, Ana M. B. .

LANCET INFECTIOUS DISEASES, 2016, 16 (06) :653-660

[9]

Campanati L., 2016, BIORXIV, V1, P25

[10] Guillain-Barre Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study [J].

Cao-Lormeau, Van-Mai ;

Blake, Alexandre ;

Mons, Sandrine ;

Lastere, Stephane ;

Roche, Claudine ;

Vanhomwegen, Jessica ;

Dub, Timothee ;

Baudouin, Laure ;

Teissier, Anita ;

Larre, Philippe ;

Vial, Anne-Laure ;

Decam, Christophe ;

Choumet, Valerie ;

Halstead, Susan K. ;

Willison, Hugh J. ;

Musset, Lucile ;

Manuguerra, Jean-Claude ;

Despres, Philippe ;

Fournier, Emmanuel ;

Mallet, Henri-Pierre ;

Musso, Didier ;

Fontanet, Arnaud ;

Neil, Jean ;

Ghawche, Frederic .

LANCET, 2016, 387 (10027) :1531-1539

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