Genotype evaluation of cowpea seeds (Vigna unguiculata) using 1H qNMR combined with exploratory tools and solid-state NMR

被引：9
作者：
Alves Filho, Elenilson G. [1 ,2 ]
Silva, Lorena M. A. [1 ]
Teofilo, Elizita M. [3 ]
Larsen, Flemming H. [4 ]
de Brito, Edy S. [1 ]
机构：
[1] EMBRAPA Agroind Trop, Fortaleza, CE, Brazil
[2] Univ Fed Ceara, Dept Food Technol, LABIOTEC, Fortaleza, Ceara, Brazil
[3] Univ Fed Ceara, Ctr Agr Sci, Fortaleza, CE, Brazil
[4] Univ Copenhagen, Dept Food Sci, DK-1168 Copenhagen, Denmark
来源：
FOOD RESEARCH INTERNATIONAL | 2017年 / 91卷
关键词：
Cowpea seeds genotype; Oligosaccharides; NMR; Chemometrics; CP-MAS; SP/MAS; PERFORMANCE LIQUID-CHROMATOGRAPHY; H-1-NMR SPECTROSCOPY; CROSS-POLARIZATION; MAS NMR; FERMENTATION; CHEMOMETRICS; JUICE; DIGESTIBILITY; ANTHOCYANINS; OLIGOSPORUS;
D O I：
10.1016/j.foodres.2016.12.007
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The ultimate aim of this study was to apply a non-targeted chemometric analysis (principal component analysis and hierarchical clustering analysis using the heat map approach) of NMR data to investigate the variability of organic compounds in nine genotype cowpea seeds, without any complex pre-treatment. In general, both exploratory tools show that Tvu 233, CE-584, and Setentao genotypes presented higher amount mainly of raffinose and Tvu 382 presented the highest content of choline and least content of raffinose. The evaluation of the aromatic region showed the Setentao genotype with highest content of niacin/vitamin B3 whereas Tvu 382 with lowest amount. To investigate rigid and mobile components in the seeds cotyledon, C-13 CP and SP/MAS solid-state NMR experiments were performed. The cotyledon of the cowpea comprised a rigid part consisting of starch as well as a soft portion made of starch, fatty acids, and protein. The variable contact time experiment suggests the presence of lipid-amylose complexes. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：140 / 147
页数：8
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