The effect of mannosylation of liposome-encapsulated indocyanine green on imaging of sentinel lymph node

被引：34

作者：

Jeong, Hwan-Seok ^{[1
,2
,3
,4
,5
]}

Lee, Chang-Moon ^{[6
]}

Cheong, Su-Jin ^{[1
,2
,3
,4
,5
]}

Kim, Eun-Mi ^{[1
,2
,3
,4
,5
]}

Hwang, Hyosook ^{[1
,2
,3
,4
,5
]}

Na, Kyung Sook ^{[1
,2
,3
,4
,5
]}

Lim, Seok Tae ^{[1
,2
,3
,4
,5
]}

Sohn, Myung-Hee ^{[1
,2
,3
,4
,5
]}

Jeong, Hwan-Jeong ^{[1
,2
,3
,4
,5
]}

机构：

[1] Chonbuk Natl Univ Med Sch & Hosp, Dept Nucl Med, Jeonju, Jeonbuk, South Korea

[2] Chonbuk Natl Univ Med Sch & Hosp, Biomed Res Inst, Jeonju, Jeonbuk, South Korea

[3] Chonbuk Natl Univ Med Sch & Hosp, Cyclotron Res Ctr, Jeonju, Jeonbuk, South Korea

[4] Chonbuk Natl Univ Med Sch & Hosp, Inst Med Sci, Jeonju, Jeonbuk, South Korea

[5] Chonbuk Natl Univ Med Sch & Hosp, Mol Imaging & Therapeut Med Res Ctr, Jeonju, Jeonbuk, South Korea

[6] Chonnam Natl Univ, Dept Biomed Engn, Yeosu, Jeonnam, South Korea

来源：

JOURNAL OF LIPOSOME RESEARCH | 2013年 / 23卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Indocyanine green; liposome; mannosylation; sentinel lymph node; FLUORESCENCE; MACROPHAGES; DELIVERY; NANOPARTICLES; DOXORUBICIN; SYSTEM; LECTIN; CELLS;

D O I：

10.3109/08982104.2013.801488

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The imaging of sentinel lymph nodes (SLN) has been researched for its role in assessing cancer progression and postsurgical lymphedema. Indocyanine green (ICG) is a near-infrared (NIR) optical dye that has been approved by the Food and Drug Administration. It is known that liposome-encapsulated ICG (LP-ICG) has improved stability and fluorescence signal compared with ICG. We designed mannosylated liposome-encapsulated ICG (M-LP-ICG) as an optical contrast agent for SLN. M-LP-ICG has a higher UV absorbance spectrum and fluorescence intensity than LP-ICG. The stability of M-LP-ICG measured in 50% fetal bovine serum solution by a dialysis method was better than that of LP-ICG. M-LP-ICG demonstrated a high uptake in RAW 264.7 macrophage cell because the density of mannose is high. There were differences between M-LP-ICG and glucosylated liposome-encapsulated ICG (G-LP-ICG), which are geometrical isomers. The result of an inhibition study of M-LP-ICG showed a statistically significant decrease in uptake in RAW 264.7 cells after either co-treatment or pre-treatment with D-(+)-mannose as an inhibitor. Results from an in vitro experiment demonstrated that M-LP-ICG was specifically taken up by macrophage cells through the mannose receptor on its surface. The time-series images acquired from a normal mouse model after subcutaneous injection showed that the signal from M-LP-ICG in SLN and other organs appeared early and disappeared quickly in comparison with signals from LP-ICG. Not only the sentinel but also the draining lymph nodes were observed partly in M-LP-ICG. M-LP-ICG appears to increase the specificity of uptake and retention in macrophages, making it a good candidate contrast agent for an optic imaging system for SLN and the lymphatic system.

引用

页码：291 / 297

页数：7

共 36 条

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