Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope

被引：158

作者：

Hofmann, UG ^{[1
]}

Rotsch, C ^{[1
]}

Parak, WJ ^{[1
]}

Radmacher, M ^{[1
]}

机构：

[1] UNIV MUNICH, LEHRSTUHL ANGEW PHYS, D-80799 MUNICH, GERMANY

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1997年 / 119卷 / 02期

关键词：

D O I：

10.1006/jsbi.1997.3868

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated living chicken cardiocytes with an atomic force microscope (AFM). Cytoskeletal structures like stress fibers can easily be imaged with the AFM. Here we have also measured the cell's elastic properties. By taking force curves as a function of lateral position (force mapping) we could compare the elastic properties at different locations of the same cell. In the lamellipodal region investigated here in detail, the elastic moduli range from around 10 up to 200 kPa on top of stress fibers. By degradation with cytochalasin B we can estimate to what extent the elastic properties of this type of cell are determined by the actin network. (C) 1997 Academic Press.

引用

页码：84 / 91

页数：8

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