Isolation and characterization of a new bacillary phytase

被引：4
作者：
Akhmetova, A. I. [1 ]
Nyamsuren, Ch. [1 ]
Balaban, N. P. [1 ]
Sharipova, M. R. [1 ]
机构：
[1] Kazan Volga Reg Fed Univ, Kazan 420008, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2013年 / 39卷 / 04期
关键词：
bacilli; phytase; isolation; purification; properties; THERMOSTABLE PHYTASE; PURIFICATION;
D O I：
10.1134/S1068162013040031
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
From cell lysates of a recombinant Escherichia coli strain, Bacillus ginsengihumi phytase has been isolated and studied for the first time. The enzyme has been purified to homogeneity, and its primary structure has been determined. It has been concluded that phytase belongs to the class of beta-propeller phosphatases. The molecular weight of the protein is 41 kDa, and pI is 4.8. Some physicochemical properties of the enzyme have been investigated.
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收藏
页码：384 / 389
页数：6
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