Phosphorylated Tyr142 β-catenin localizes to centrosomes and is regulated by Syk

beta-catenin is a central component of adherent junctions and a key effector of canonical Wnt signaling, in which dephosphorylated Ser/Thr -catenin regulates gene transcription. -catenin phosphorylation at Tyr142 (PTyr142 -catenin), which is induced by receptor and Src family Tyr kinases, represents a previously described -catenin switch from adhesive to migratory roles. In addition to classical -catenin roles, phosphorylated Ser/Thr -catenin and total -catenin were involved in centrosomal functions, including mitotic spindle formation and centrosome separation. Here we find that PTyr142 -catenin is present in centrosomes in non-transformed and glioblastoma cells and that, in contrast to the Ser/Thr phosphorylated -catenin, PTyr142 -catenin centrosomal levels drop in mitosis. Furthermore, we show that the inhibitor of Spleen Tyrosine Kinase (Syk) piceatannol decreases centrosomal PTyr142 -catenin levels, indicating that Syk regulates centrosome PTyr142 -catenin. Our findings suggest that PTyr142 -catenin and Syk may regulate centrosomal cohesion. This study highlights the contribution of different phosphorylated -catenin forms to the cell and centrosome cycles.