Blotting Assisted by Heating and Solvent Extraction for DESI-MS Imaging

被引：71
作者：
Cabral, Elaine C. [1 ]
Mirabelli, Mario F. [1 ,2 ]
Perez, Consuelo J. [1 ]
Ifa, Demian R. [1 ]
机构：
[1] York Univ, Ctr Res Mass Spectrometry, Toronto, ON M3J 2R7, Canada
[2] Univ Calabria, I-87036 Cosenza, Italy
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2013年 / 24卷 / 06期
关键词：
Mass spectrometry imaging; Desorption electrospray ionization; Ambient ionization; IONIZATION MASS-SPECTROMETRY; GINKGO-BILOBA; POTATO GLYCOALKALOIDS; QUANTITATIVE-ANALYSIS; TOF MS; TISSUE; MATRIX; BIFLAVONES; STRAWBERRY; IDENTIFICATION;
D O I：
10.1007/s13361-013-0616-y
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Imprints of potato sprout (Solanum tuberosum L.), gingko leaves (Gingko biloba L.) and strawberries (Fragaria x ananassa Duch.) were successfully imaged by desorption electrospray ionization mass spectrometry (DESI-MS) on TLC plates through blotting assisted by heating and/or solvent extraction. Ion images showing the distribution of significant compounds such as glycoalkaloid toxins in potato sprout, ginkgolic acids and flavonoids in ginkgo leaves, and sugars and anthocyanidin in strawberry were obtained. Practical implications of this work include analysis of a wide range of irregular or soft materials by different imprinting conditions without requiring the addition of matrices or use of specific kinds of surfaces.
引用
收藏
页码：956 / 965
页数：10
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