The advantage of automatic control in human-like collagen production by recombinant Escherichia coli BL21

Two control models, manual way and automatic way, have been compared during the fermentation process in this study. The automatic way is achieved by a fermentation controller developed by ourselves. Compared to manual way, biomass (OD600) and the production of human-like collagen (HLC) could be increases significantly, reaching to 144 and 7.12g/L respectively. Acetic acid, a key byproduct, is also lowered very obviously. Beside the production controlled by automation model was very stable, the automatic controller can regulate the feeding rate in real time and save labor force.