Component analysis of extracellular polymeric substances (EPS) during aerobic sludge granulation using FTIR and 3D-EEM technologies

被引：366

作者：

Zhu, Liang ^{[1
]}

Qi, Han-ying ^{[1
]}

Lv, Mei-le ^{[1
]}

Kong, Yun ^{[1
]}

Yu, Yan-wen ^{[1
]}

Xu, Xiang-yang ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Dept Environm Engn, Hangzhou 310058, Zhejiang, Peoples R China

[2] ZJU UWA Joint Ctr Integrated Water Management & P, Hangzhou 310058, Zhejiang, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 124卷

基金：

中国国家自然科学基金;

关键词：

Extracellular polymeric substances (EPS); Aerobic granule; Component analysis; Three-dimensional fluorescence spectrum technology (3D-EEM); Fourier transform infrared (FTIR); ORGANIC-MATTER FRACTIONS; PHOSPHORUS REMOVAL; SHEAR FORCE; GRANULES; SPECTROSCOPY; MATRIX; EXCITATION; PROTEINS; DOM;

D O I：

10.1016/j.biortech.2012.08.059

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In recent years, lots of the extracellular polymeric substances (EPS) related researches have focused on its role in the granulation and structural stability of aerobic sludge. Three-dimensional fluorescence spectrum (3D-EEM) and fourier transform infrared spectroscopy (FTIR) technologies were used to analyse the main components of sludge EPS during aerobic sludge granulation in this study. Results showed that the components of sludge EPS tended to be stable during aerobic sludge granulation. The peak F (Ex/Em = 230/308.5) from 3D-EEM and the predominant spectral band at approximately 1517 cm(-1) from the FTIR spectra of the matured granular sludge indicated the importance of aromatic protein-like substances together, especially tyrosine in maintaining the stable structure of the granular sludge. Furthermore, the differences in the occurrence position and frequency of C-O bonds (1110-1047 cm(-1)) observed during aerobic sludge granulation showed that the transformations between the isomers and other forms of carbohydrates may be attributed to the formation of aerobic granule. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：455 / 459

页数：5

共 35 条

[1] Aerobic granular sludge: Recent advances [J].

Adav, Sunil S. ;

Lee, Duu-Jong ;

Show, Kuan-Yeow ;

Tay, Joo-Hwa .

BIOTECHNOLOGY ADVANCES, 2008, 26 (05) :411-423

[2] Extracellular polymeric substances and structural stability of aerobic granule [J].

Adav, Sunil S. ;

Lee, Duu-Jong ;

Tay, Joo-Hwa .

WATER RESEARCH, 2008, 42 (6-7) :1644-1650

[3]

[Anonymous], 2006, STANDARD METHODS EXA, DOI DOI 10.5860/CHOICE.37-2792

[4] Role of extracellular polymeric substances in bioflocculation of activated sludge microorganisms under glucose-controlled conditions [J].

Badireddy, Appala R. ;

Chellam, Shankararaman ;

Gassman, Paul L. ;

Engelhard, Mark H. ;

Lea, Alan S. ;

Rosso, Kevin M. .

WATER RESEARCH, 2010, 44 (15) :4505-4516

[5] What vibrations tell us about proteins [J].

Barth, A ;

Zscherp, C .

QUARTERLY REVIEWS OF BIOPHYSICS, 2002, 35 (04) :369-430

[6] COMPARISON OF BACTERIAL EXTRACELLULAR POLYMER EXTRACTION METHODS [J].

BROWN, MJ ;

LESTER, JN .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1980, 40 (02) :179-185

[7] Spectroscopic characterization of the structural and functional properties of natural organic matter fractions [J].

Chen, J ;

Gu, BH ;

LeBoeuf, EJ ;

Pan, HJ ;

Dai, S .

CHEMOSPHERE, 2002, 48 (01) :59-68

[8] Distribution of extracellular polymeric substances in aerobic granules [J].

Chen, M. Y. ;

Lee, D. J. ;

Tay, J. H. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 73 (06) :1463-1469

[9] Fluorescence excitation - Emission matrix regional integration to quantify spectra for dissolved organic matter [J].

Chen, W ;

Westerhoff, P ;

Leenheer, JA ;

Booksh, K .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2003, 37 (24) :5701-5710

[10] Characterization of marine and terrestrial DOM in seawater using excitation emission matrix spectroscopy [J].

Coble, PG .

MARINE CHEMISTRY, 1996, 51 (04) :325-346

← 1 2 3 4 →