Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis

被引：48

作者：

Fernandes, Felipe H. A. ^{[1
]}

Batista, Rayanne S. de A. ^{[2
]}

de Medeiros, Francinalva D. ^{[1
]}

Santos, Fbio S. ^{[2
]}

Medeiros, Ana C. D. ^{[1
]}

机构：

[1] Univ Estadual Paraiba, Ctr Ciencias Biol & Saude, Lab Desenvolvimento & Ensaios Medicamentos, Campina Grande, PB, Brazil

[2] Univ Fed Paraiba, Lab Unificados Desenvolvimento & Ensaios Medicame, Dept Ciencias Farmaceut, Ctr Ciencias Saude, BR-58059900 Joao Pessoa, Paraiba, Brazil

来源：

REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2015年 / 25卷 / 03期

关键词：

Gallic acid; Chemical marker; Validation; Schinopsis brasiliensis; ANALYTICAL METHODOLOGIES; PHENOLIC-COMPOUNDS; VALIDATION; QUANTIFICATION; ANTIOXIDANT; FLAVONOIDS; LEAVES; ENGL;

D O I：

10.1016/j.bjp.2015.05.006

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250 x 4.6 mm, 5 mu m). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1 ml/min and effluents were monitored at 271 nm. The retention time for gallic acid was 8.5 min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis. (C) 2015 Published by Elsevier Editora Ltda. on behalf of Sociedade Brasileira de Farmacognosia.

引用

页码：208 / 211

页数：4

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