Isolation, characterization and cross-species amplification of microsatellite DNA loci in the tropical American yam Dioscorea trifida

被引：19
|
作者：
Hochu, I
Santoni, S
Bousalem, M [1 ]
机构：
[1] URPV, INRA, Domaine Duclos,Prise Eau, F-97170 Petit Bourg, Guadeloupe, France
[2] INRA, Stn Ameliorat Plantes, F-34060 Montpellier, France
来源：
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 01期
关键词：
cross-species amplification; cultivated yam; Dioscorea trifida; microsatellites;
D O I：
10.1111/j.1471-8286.2005.01166.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed microsatellite markers in American yam (Dioscorea trifida). A microsatellite sequence-enriched genomic library was screened, and after sequencing, primers were designed for 20 microsatellites. Among these, eight primer pairs yielded amplification products that were both interpretable and polymorphic in 24 yam cultivars. The number of alleles per locus ranged from two to 13 and the overall expected heterozygosity was around 0.5. Six of the eight Dioscorea trifida microsatellite loci gave amplification products in other Dioscorea species.
引用
收藏
页码：137 / 140
页数：4
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