Single-cell analysis on stromal fibroblasts in the microenvironment of solid tumours

被引：18

作者：

Musa, Marahaini ^{[1
]}

机构：

[1] Univ Sains Malaysia, Human Genome Ctr, Sch Med Sci, Hlth Campus, Kubang Kerian 16150, Kelantan, Malaysia

来源：

ADVANCES IN MEDICAL SCIENCES | 2020年 / 65卷 / 01期

关键词：

Single-cell analysis; Cancer; Stromal fibroblast; Solid tumour; Microenvironment; CANCER-ASSOCIATED FIBROBLASTS; GENE-EXPRESSION; RNA-SEQ; MASS CYTOMETRY; STEM-CELLS; HETEROGENEITY; PROGRESSION; MYOFIBROBLASTS; VISUALIZATION; LEUKEMIA;

D O I：

10.1016/j.advms.2019.12.001

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Besides malignant cells, the tumour microenvironment consists of various stromal cells such as cancer-associated fibroblasts (CAFs) and myofibroblasts. Accumulation of heterogeneous populations of stromal cells in solid tumours is associated with lower survival rates and cancer recurrence in patients. Certain limitations presented by conventional experimental designs and techniques in cancer research have led to poor understanding of the fundamental basis of cancer niche. Recent developments in single-cell techniques allow more in-depth studies of the tumour microenvironment. Analyses at the single-cell level enables the detection of rare cell types, characterization of intra-tumour cellular heterogeneity and analysis of the lineage output of malignant cells. This subsequently, provides valuable insights on better diagnostic methods and treatment avenues for cancer. This review explores the recent advancements and applications of single-cell technologies in cancer research pertaining to the study of stromal fibroblasts in the microenvironment of solid tumours.

引用

页码：163 / 169

页数：7

共 90 条

[41] Analysis of Single-Cell RNA-Seq Identifies Cell-Cell Communication Associated with Tumor Characteristics [J].

Kumar, Manu P. ;

Du, Jinyan ;

Lagoudas, Georgia ;

Jiao, Yang ;

Sawyer, Andrew ;

Drummond, Daryl C. ;

Lauffenburger, Douglas A. ;

Raue, Andreas .

CELL REPORTS, 2018, 25 (06) :1458-+

[42] Reconstruction of functionally normal and malignant human breast tissues in mice [J].

Kuperwasser, C ;

Chavarria, T ;

Wu, M ;

Magrane, G ;

Gray, JW ;

Carey, L ;

Richardson, A ;

Weinberg, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (14) :4966-4971

[43] Phenotype molding of stromal cells in the lung tumor microenvironment [J].

Lambrechts, Diether ;

Wauters, Els ;

Boeckx, Bram ;

Aibar, Sara ;

Nittner, David ;

Burton, Oliver ;

Bassez, Ayse ;

Decaluwe, Herbert ;

Pircher, Andreas ;

Van den Eynde, Kathleen ;

Weynand, Birgit ;

Verbeken, Erik ;

De Leyn, Paul ;

Liston, Adrian ;

Vansteenkiste, Johan ;

Carmeliet, Peter ;

Aerts, Stein ;

Thienpont, Bernard .

NATURE MEDICINE, 2018, 24 (08) :1277-+

[44] Molecular mechanisms of epithelial-mesenchymal transition [J].

Lamouille, Samy ;

Xu, Jian ;

Derynck, Rik .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2014, 15 (03) :178-196

[45] Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells [J].

Lapin, Morten ;

Tjensvoll, Kjersti ;

Oltedal, Satu ;

Javle, Milind ;

Smaaland, Rune ;

Gilje, Bjornar ;

Nordgard, Oddmund .

BMC CANCER, 2017, 17

[46] Mutational heterogeneity in cancer and the search for new cancer-associated genes [J].

Lawrence, Michael S. ;

Stojanov, Petar ;

Polak, Paz ;

Kryukov, Gregory V. ;

Cibulskis, Kristian ;

Sivachenko, Andrey ;

Carter, Scott L. ;

Stewart, Chip ;

Mermel, Craig H. ;

Roberts, Steven A. ;

Kiezun, Adam ;

Hammerman, Peter S. ;

McKenna, Aaron ;

Drier, Yotam ;

Zou, Lihua ;

Ramos, Alex H. ;

Pugh, Trevor J. ;

Stransky, Nicolas ;

Helman, Elena ;

Kim, Jaegil ;

Sougnez, Carrie ;

Ambrogio, Lauren ;

Nickerson, Elizabeth ;

Shefler, Erica ;

Cortes, Maria L. ;

Auclair, Daniel ;

Saksena, Gordon ;

Voet, Douglas ;

Noble, Michael ;

DiCara, Daniel ;

Lin, Pei ;

Lichtenstein, Lee ;

Heiman, David I. ;

Fennell, Timothy ;

Imielinski, Marcin ;

Hernandez, Bryan ;

Hodis, Eran ;

Baca, Sylvan ;

Dulak, Austin M. ;

Lohr, Jens ;

Landau, Dan-Avi ;

Wu, Catherine J. ;

Melendez-Zajgla, Jorge ;

Hidalgo-Miranda, Alfredo ;

Koren, Amnon ;

McCarroll, Steven A. ;

Mora, Jaume ;

Lee, Ryan S. ;

Crompton, Brian ;

Onofrio, Robert .

NATURE, 2013, 499 (7457) :214-218

[47] Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors [J].

Li, Huipeng ;

Courtois, Elise T. ;

Sengupta, Debarka ;

Tan, Yuliana ;

Chen, Kok Hao ;

Goh, Jolene Jie Lin ;

Kong, Say Li ;

Chua, Clarinda ;

Hon, Lim Kiat ;

Tan, Wah Siew ;

Wong, Mark ;

Choi, Paul Jongjoon ;

Wee, Lawrence J. K. ;

Hillmer, Axel M. ;

Tan, Iain Beehuat ;

Robson, Paul ;

Prabhakar, Shyam .

NATURE GENETICS, 2017, 49 (05) :708-+

[48] Bifurcation analysis of single-cell gene expression data reveals epigenetic landscape [J].

Marco, Eugenio ;

Karp, Robert L. ;

Guo, Guoji ;

Robson, Paul ;

Hart, Adam H. ;

Trippa, Lorenzo ;

Yuan, Guo-Cheng .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (52) :E5643-E5650

[49] Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions [J].

Massani, Marco ;

Stecca, Tommaso ;

Fabris, Luca ;

Caratozzolo, Ezio ;

Ruffolo, Cesare ;

Furlanetto, Alberto ;

Morton, Stuart ;

Cadamuro, Massimiliano ;

Strazzabosco, Mario ;

Bassi, Nicolo .

ONCOLOGY REPORTS, 2013, 30 (03) :1143-1148

[50] Decoding the regulatory network of early blood development from single-cell gene expression measurements [J].

Moignard, Victoria ;

Woodhouse, Steven ;

Haghverdi, Laleh ;

Lilly, Andrew J. ;

Tanaka, Yosuke ;

Wilkinson, Adam C. ;

Buettner, Florian ;

Macaulay, Iain C. ;

Jawaid, Wajid ;

Diamanti, Evangelia ;

Nishikawa, Shin-Ichi ;

Piterman, Nir ;

Kouskoff, Valerie ;

Theis, Fabian J. ;

Fisher, Jasmin ;

Goettgens, Berthold .

NATURE BIOTECHNOLOGY, 2015, 33 (03) :269-+

← 1 2 3 4 5 6 7 8 9 →