Hyphenation of fast liquid chromatography with high-resolution mass spectrometry for quantitative proteomics analyses

被引：11

作者：

Lesur, Antoine ^{[1
]}

Gallien, Sebastien ^{[1
]}

Domon, Bruno ^{[1
]}

机构：

[1] Luxembourg Inst Hlth, Luxembourg Clin Prote Ctr, 1 A-B Rue Thomas Edison, L-1445 Strassen, Luxembourg

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2016年 / 84卷

关键词：

Target proteomics; Ultra UHPLC; Isotopically labeled peptides; Internal standard; High-resolution mass spectrometry; PRM (Parallel Reaction Monitoring); Orbitrap mass spectrometry; TARGETED PROTEOMICS; QUANTIFICATION; QUADRUPOLE; PROTEINS; TIME; PRM; MS;

D O I：

10.1016/j.trac.2016.01.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Targeted analyses performed on high-resolution accurate mass spectrometers hyphenated to liquid chromatographs have opened new opportunities in quantitative proteomics. A new extension of the parallel reaction monitoring (PRM) technique uses the internal standards added to samples to dynamically control the data acquisition and maximize the analysis of the endogenous peptides. This account describes the hyphenation of ultra-HPLC with a high acquisition rate quadrupole-orbitrap instrument to properly sample the sharp elution profiles of UHPLC separations, possible with the IS-PRM technique. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：144 / 150

页数：7

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