Genomic characterization of liver metastases from colorectal cancer patients

被引：71

作者：

Maria Sayagues, Jose ^{[1
,2
]}

Antonio Corchete, Luis ^{[3
,4
]}

Laura Gutierrez, Maria ^{[1
,2
]}

Eugenia Sarasquete, Maria ^{[3
,4
]}

del Mar Abad, Maria ^{[5
]}

Bengoechea, Oscar ^{[5
]}

Ferminan, Encarna ^{[6
]}

Fernanda Anduaga, Maria ^{[7
,8
]}

del Carmen, Sofia ^{[5
]}

Iglesias, Manuel ^{[7
,8
]}

Esteban, Carmen ^{[7
,8
]}

Angoso, Maria ^{[7
,8
]}

Antonio Alcazar, Jose ^{[7
,8
]}

Garcia, Jacinto ^{[7
,8
]}

Orfao, Alberto ^{[1
,2
]}

Munoz-Bellvis, Luis ^{[7
,8
]}

机构：

[1] USAL, CSIC, IBMCC, Cytometry Serv NUCLEUS,Dept Med,Canc Res Ctr, Salamanca, Spain

[2] IBSAL Univ Salamanca, Salamanca, Spain

[3] Univ Hosp Salamanca, Ctr Canc Res, Salamanca, Spain

[4] Univ Hosp Salamanca, Serv Hematol, Salamanca, Spain

[5] Univ Hosp Salamanca, Dept Pathol, Salamanca, Spain

[6] USAL, CSIC, IBMCC, Genom Unit,Canc Res Ctr, Salamanca, Spain

[7] Univ Hosp Salamanca, Serv Gen & Gastrointestinal Surg, Salamanca, Spain

[8] Univ Hosp Salamanca, IBSAL, Salamanca, Spain

来源：

ONCOTARGET | 2016年 / 7卷 / 45期

关键词：

GEP; colorectal cancer; INDEPENDENT PROGNOSTIC-FACTOR; EXPRESSION; CELLS; PROGRESSION; GROWTH; IDENTIFICATION; BIOMARKERS; INVASION; PATHWAYS; PROMOTES;

D O I：

10.18632/oncotarget.12140

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Metastatic dissemination is the most frequent cause of death of sporadic colorectal cancer (sCRC) patients. Genomic abnormalities which are potentially characteristic of such advanced stages of the disease are complex and so far, they have been poorly described and only partially understood. We evaluated the molecular heterogeneity of sCRC tumors based on simultaneous assessment of the overall GEP of both coding mRNA and non-coding RNA genes in primary sCRC tumor samples from 23 consecutive patients and their paired liver metastases. Liver metastases from the sCRC patients analyzed, systematically showed deregulated transcripts of those genes identified as also deregulated in their paired primary colorectal carcinomas. However, some transcripts were found to be specifically deregulated in liver metastases (vs. non-tumoral colorectal tissues) while expressed at normal levels in their primary tumors, reflecting either an increased genomic instability of metastatic cells or theiradaption to the liver microenvironment. Newly deregulated metastatic transcripts included overexpression of APOA1, HRG, UGT2B4, RBP4 and ADH4 mRNAS and the miR-3180-3p, miR-3197, miR-3178, miR-4793 and miR-4440 miRNAs, together with decreased expression of the IGKV1-39, IGKC, IGKV1-27, FABP4 and MYLK mRNAS and the miR-363, miR-1, miR-143, miR-27b and miR-28-5p miRNAs. Canonical pathways found to be specifically deregulated in liver metastatic samples included multiple genes related with intercellular adhesion and the metastatic processes (e.g., IGF1R, PIK3CA, PTEN and EGFR), endocytosis (e.g., the PDGFRA, SMAD2, ERBB3, PML and FGFR2), and the cell cycle (e.g., SMAD2, CCND2, E2F5 and MYC). Our results also highlighted the activation of genes associated with the TGF beta signaling pathway, -e.g. RHOA, SMAD2, SMAD4, SMAD5, SMAD6, BMPR1A, SMAD7 and MYC-, which thereby emerge as candidate genes to play an important role in CRC tumor metastasis.

引用

页码：72908 / 72922

页数：15

共 61 条

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