Apoptin Induces Apoptosis in Human Bladder Cancer EJ and BIU-87 Cells

被引：3
作者：
Zhan, Hui [1 ]
Wang, Jian-Song [1 ]
Wang, Hai-Feng [1 ]
Zuo, Yi-Gang [1 ]
Wang, Chun-Hui [1 ]
Ding, Ming-Xia [1 ]
机构：
[1] Kunming Med Univ, Affiliated Hosp 2, Dept Urol, Kunming, Peoples R China
来源：
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2012年 / 13卷 / 01期
关键词：
Apoptin; urinary bladder neoplasms; apoptosis; therapy; CHICKEN ANEMIA VIRUS; NUCLEAR-LOCALIZATION; SIGNAL;
D O I：
10.7314/APJCP.2012.13.1.135
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objective: To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy. Methods: We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method). Results: PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p < 0.05). Conclusions: Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.
引用
收藏
页码：135 / 138
页数：4
相关论文
共 22 条
  • [1] Apoptin: Therapeutic potential of an early sensor of carcinogenic transformation
    Backendorf, Claude
    Visser, Astrid E.
    de Boer, A. G.
    Zliumerman, Rhyenne
    Visser, Mijke
    Voskamp, Patrick
    Zhang, Ying-Hui
    Noteborn, Matlueu
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2008, 48 : 143 - 169
  • [2] Human death effector domain-associated factor interacts with the viral apoptosis agonist Apoptin and exerts tumor-preferential cell killing
    Danen-van Oorschot, AAAM
    Voskamp, P
    Seelen, MCMJ
    van Miltenburg, MHAM
    Bolk, MW
    Tait, SW
    Boesen-de Cock, JGR
    Rohn, JL
    Borst, J
    Noteborn, MHM
    [J]. CELL DEATH AND DIFFERENTIATION, 2004, 11 (05) : 564 - 573
  • [3] Importance of nuclear localization of apoptin for tumor-specific induction of apoptosis
    Danen-van Oorschot, AAAM
    Zhang, YH
    Leliveld, SR
    Rohn, JL
    Seelen, MCMJ
    Bolk, MW
    van Zon, A
    Erkeland, SJ
    Abrahams, JP
    Mumberg, D
    Noteborn, MHM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (30) : 27729 - 27736
  • [4] Gu FL, 2003, CHINESE MED J-PEKING, V116, P1391
  • [5] Apoptin sensitizes radiation-induced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells
    Han, Su-Xia
    Zhu, Qing
    Ma, Jin-Lu
    Lv, Yi
    Zhao, Jing
    Huang, Chen
    Jia, Xi
    Ou, Wei
    Guo, Hong-Tao
    [J]. MOLECULAR MEDICINE REPORTS, 2011, 4 (01) : 59 - 63
  • [6] Han Su-xia, 2010, Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, V26, P310
  • [7] Han Su-xia, 2008, Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, V24, P864
  • [8] Apoptin's functional N- and C-termini independently bind DNA
    Leliveld, SR
    Dame, RT
    Rohn, JL
    Noteborn, MHM
    Abrahams, JP
    [J]. FEBS LETTERS, 2004, 557 (1-3) : 155 - 158
  • [9] Apoptin acts as a tumor-specific killer: Potentials for an anti-tumor therapy
    Noteborn, MHM
    [J]. CELLULAR AND MOLECULAR BIOLOGY, 2005, 51 (01) : 49 - 60
  • [10] Chicken anemia virus induced apoptosis: underlying molecular mechanisms
    Noteborn, MHM
    [J]. VETERINARY MICROBIOLOGY, 2004, 98 (02) : 89 - 94
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