Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum

被引：51

作者：

Chen, Tao ^{[1
]}

Liu, Yongling ^{[1
,2
]}

Zou, Denglang ^{[1
,2
]}

Chen, Chen ^{[1
]}

You, Jinmao ^{[1
]}

Zhou, Guoying ^{[1
]}

Sun, Jing ^{[1
]}

Li, Yulin ^{[1
]}

机构：

[1] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2014年 / 37卷 / 1-2期

关键词：

Anthraquinones; High-speed counter-current chromatography; Liquid-liquid extraction; Preparative HPLC; Rheum tanguticum; STILBENE GLYCOSIDES; CONSTITUENTS; PERFORMANCE; CCC;

D O I：

10.1002/jssc.201300648

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.

引用

页码：165 / 170

页数：6

共 25 条

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