Exogenous nitric oxide modulates cytokine production in human leukocytes

被引：14

作者：

Kallmann, BA ^{[1
]}

Malzkorn, R ^{[1
]}

Kolb, H ^{[1
]}

机构：

[1] Univ Dusseldorf, Diabet Res Inst, D-40225 Dusseldorf, Germany

来源：

LIFE SCIENCES | 1999年 / 65卷 / 17期

关键词：

nitric oxide; cytokines; human leukocytes;

D O I：

10.1016/S0024-3205(99)00431-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Exogenous nitric oxide was found to modify the pattern of cytokine secretion from human leukocytes, with similar outcome in 11 different healthy blood donors. Peripheral blood mononuclear cells (PBMC) were stimulated with phytohaemagglutinin (PHA) in the presence of increasing amounts of the NO donor S-nitroso-N-acetyl-penicillamine (SNAP). The NO donor dose-dependently enhanced IL-4 secretion into the supernatant (p< 0.01). In contrast, IFN gamma production was not affected while IL-10 levels were slightly decreased. Comparable changes were observed when analysing cytokine mRNA levels by semiquantiative RT-PCR. The differential effect of the NO donor on IL-4 versus IL-10 and IFN gamma gene expression suggests an immunomodulatory potential of NO, which may serve to limit inflammatory responses.

引用

页码：1787 / 1794

页数：8

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