Contamination of milk with Bacillus cereus by post-pasteurization surface exposure as evaluated by automated ribotyping

被引:47
作者
Salustiano, V. C. [1 ]
Andrade, N. J. [1 ]
Soares, N. F. F. [1 ]
Lima, J. C. [1 ]
Bernardes, P. C. [1 ]
Luiz, L. M. P. [1 ]
Fernandes, P. E. [1 ]
机构
[1] Univ Fed Vicosa, Dept Food Technol, BR-36570000 Vicosa, MG, Brazil
关键词
Milk quality; Milk recontamination; Bacillus cereus; Ribotyping; BIOFILMS;
D O I
10.1016/j.foodcont.2008.07.004
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Over the last decade, increased transportation of refrigerated raw milk from farms to factories has raised concerns over Bacillus cereus contamination in the Brazilian dairy industry. Twelve isolates from pasteurized milk and 30 isolates from the post-pasteurization equipment Surfaces of a dairy processing unit were characterized as B. cereus. Seven ribotypes were identified, demonstrating the genetic variability of this microorganism. Most of the isolates belonged to the same ribogroup (RIBO1222-73-S4). and they were found on four surfaces and also in the milk, indicating the role of the equipment surfaces as reservoirs for milk recontamination. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:439 / 442
页数:4
相关论文
共 19 条
[1]   Comparison between automatic ribotyping and random amplified polymorphic DNA analysis of Bacillus cereus isolates from the dairy industry [J].
Andersson, A ;
Svensson, B ;
Christiansson, A ;
Rönner, U .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 47 (1-2) :147-151
[2]  
Bennett R.W., 2001, COMPENDIUM METHODS M, P311
[3]   ADSA Foundation Scholar Award -: Fluid dairy product quality and safety:: Looking to the future [J].
Boor, KJ .
JOURNAL OF DAIRY SCIENCE, 2001, 84 (01) :1-11
[4]  
Chmielewski R A N, 2003, Compr Rev Food Sci Food Saf, V2, P22, DOI 10.1111/j.1541-4337.2003.tb00012.x
[5]   HEAT-RESISTANT PSYCHROTROPHIC MICROORGANISMS [J].
COLLINS, EB .
JOURNAL OF DAIRY SCIENCE, 1981, 64 (01) :157-160
[6]   INFLUENCE OF PSYCHROTROPHIC BACTERIAL-GROWTH IN RAW-MILK ON THE SENSORY ACCEPTANCE OF UHT SKIM MILK [J].
COLLINS, SJ ;
BESTER, BH ;
MCGILL, AEJ .
JOURNAL OF FOOD PROTECTION, 1993, 56 (05) :418-425
[7]   BACTERIAL BIOFILMS IN NATURE AND DISEASE [J].
COSTERTON, JW ;
CHENG, KJ ;
GEESEY, GG ;
LADD, TI ;
NICKEL, JC ;
DASGUPTA, M ;
MARRIE, TJ .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :435-464
[8]   Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants [J].
Dogan, B ;
Boor, KJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (01) :130-138
[9]   Critical contamination sites in the production line of pasteurised milk, with reference to the psychrotrophic spoilage flora [J].
Eneroth, Å ;
Christiansson, A ;
Brendehaug, J ;
Molin, G .
INTERNATIONAL DAIRY JOURNAL, 1998, 8 (09) :829-834
[10]  
Evanch G M., 2001, Compendium of methods for the microbiological examination of foods, V4 th, P25