TiO2 Scaffolds Sustain Differentiation of MC3T3-E1 Cells

被引：15

作者：

Gomez-Florit, Manuel ^{[1
]}

Rubert, Marina ^{[1
]}

Ramis, Joana M. ^{[1
]}

Haugen, Havard J. ^{[2
]}

Tiainen, Hanna ^{[2
]}

Lyngstadaas, Staale Petter ^{[2
]}

Monjo, Marta ^{[1
]}

机构：

[1] Univ Balearic Isl, Dept Fundamental Biol & Hlth Sci, Res Inst Hlth Sci IUNICS, E-07122 Palma De Mallorca, Spain

[2] Univ Oslo, Dept Biomat, N-0317 Oslo, Norway

来源：

JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING | 2012年 / 2卷 / 04期

关键词：

Titanium Dioxide; Three-Dimensional Scaffold; Osteoblast; Seeding; Differentiation; Gene Expression; IN-VITRO; OSTEOBLAST DIFFERENTIATION; BONE; TISSUE; CULTURE; MATRIX; EXPRESSION; FIBRONECTIN; MINERALIZATION; PROLIFERATION;

D O I：

10.1166/jbt.2012.1055

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The aim of the present study was to investigate distribution, growth and differentiation of MC3T3-E1 osteoblasts over time using an agitated seeding method into highly porous TiO2 scaffolds. Cells penetrated into the core of the 3D scaffold structure as confirmed with confocal microscopy analysis. DNA quantification and scanning electron microscopy confirmed a good proliferation of cells on the TiO2 scaffolds. Moreover, time course of gene expression profile of cell adhesion (fibronectin and integrins) and osteoblast phenotypic markers (collagen type 1, bone morphogenetic protein 2, bone sialoprotein, osteopontin and osteocalcin) showed a good progression in the sequential stages of osteoblast differentiation, i.e., initial adhesion and proliferation, extracellular matrix maturation and mineralization. This was followed by an increase in ALP activity and calcium content after 21 days, the hallmark of osteoblast function. Taking all these results together, TiO2 scaffolds have shown to sustain MC3T3-E1 growth towards a mature and differentiated state.

引用

页码：336 / 344

页数：9

共 55 条

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