In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display

被引：23

作者：

Li, Zha ^{[1
,2
]}

Uzawa, Takanori ^{[1
]}

Tanaka, Takashi ^{[3
]}

Hida, Akira ^{[4
]}

Ishibashi, Koji ^{[4
]}

Katakura, Hiromichi ^{[3
]}

Kobatake, Eiry ^{[2
]}

Ito, Yoshihiro ^{[1
]}

机构：

[1] RIKEN, Adv Sci Inst, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

[2] Tokyo Inst Technol, Dept Biol Informat, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[3] Natl Inst Adv Ind Sci & Technol, Nanosyst Res Inst NRI, Tsukuba, Ibaraki 3058562, Japan

[4] RIKEN, Adv Sci Inst, Adv Device Lab, Wako, Saitama 3510198, Japan

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 01期

关键词：

Aptamer; In vitro selection; Peptide; Ribosome display; Single-wall carbon nanotubes; DISPERSION; PROTEIN; WATER; SOLUBILIZATION; FLUORESCENCE; CHIRALITY;

D O I：

10.1007/s10529-012-1049-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A ribosome display from a diverse random library was applied for selecting peptide aptamers with high binding affinity to single-wall carbon nanotubes (SWCNTs). The selected peptide aptamer bound to and solubilized SWCNTs more strongly than did the peptide aptamer selected by a phage display method reported previously, and more strongly than other commonly used organic surfactants. The fluorescence spectrum of this aptamer showed a red shift upon interaction with SWCNTs but circular dichroism spectroscopy did not show any significant difference between the presence or absence of SWCNT binding.

引用

页码：39 / 45

页数：7

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