Modes of correlated angular motion in live cells across three distinct time scales

被引：29

作者：

Harrison, Andrew W. ^{[1
,2
,3
]}

Kenwright, David A. ^{[1
,2
]}

Waigh, Thomas A. ^{[2
,3
]}

Woodman, Philip G. ^{[1
]}

Allan, Victoria J. ^{[1
,3
]}

机构：

[1] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England

[2] Univ Manchester, Sch Phys & Astron, Manchester M13 9PL, Lancs, England

[3] Univ Manchester, Photon Sci Inst, Manchester M13 9PL, Lancs, England

来源：

PHYSICAL BIOLOGY | 2013年 / 10卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

PARTICLE-TRACKING MICRORHEOLOGY; SUBDIFFUSION; TRANSPORT; DYNAMICS;

D O I：

10.1088/1478-3975/10/3/036002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Particle tracking experiments with high speed digital microscopy yield the positions and trajectories of lipid droplets inside living cells. Angular correlation analysis shows that the lipid droplets have uncorrelated motion at short time scales (tau < 1 ms) followed by anti-persistent motion for lag times in the range of 1 <= tau <= 10 ms. The angular correlation at longer time scales, tau > 10 ms, becomes persistent, indicating directed movement. The motion at all time scales is associated with the lipid droplets being tethered to and driven along the microtubule network. The point at which the angular correlation changes from anti-persistent to persistent motion corresponds to the cross over between sub-diffusive and super diffusive motion, as observed by mean square displacement analysis. Correct analysis of the angular correlations of the detector noise is found to be crucial in modelling the observed phenomena.

引用

页数：10

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