ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF

被引：287

作者：

Hamiche, A ^{[1
]}

Sandaltzopoulos, R ^{[1
]}

Gdula, DA ^{[1
]}

Wu, C ^{[1
]}

机构：

[1] NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

CELL | 1999年 / 97卷 / 07期

关键词：

D O I：

10.1016/S0092-8674(00)80796-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drosophila NURF is an ATP-dependent chromatin remodeling complex that contains ISWI, a member of the SW12/SNF2 family of ATPases. We demonstrate that NURF catalyzes the bidirectional redistribution of mononucleosomes reconstituted on hsp70 promoter DNA. In the presence of NURF, nucleosomes adopt one predominant position from an ensemble of possible locations within minutes. Movements occur in cis, with no transfer to competing DNA. Migrating intermediates trapped by fro III digestion reveal progressive nucleosome motion in increments of several base pairs. All four core histones are retained quantitatively during this process, indicating that the general integrity of the histone octamer is maintained. We suggest that NURF remodels nucleosomes by transiently decreasing the activation energy for short-range sliding of the histone octamer.

引用

页码：833 / 842

页数：10

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