ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF

被引：287
作者：
Hamiche, A [1 ]
Sandaltzopoulos, R [1 ]
Gdula, DA [1 ]
Wu, C [1 ]
机构：
[1] NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA
来源：
CELL | 1999年 / 97卷 / 07期
关键词：
D O I：
10.1016/S0092-8674(00)80796-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Drosophila NURF is an ATP-dependent chromatin remodeling complex that contains ISWI, a member of the SW12/SNF2 family of ATPases. We demonstrate that NURF catalyzes the bidirectional redistribution of mononucleosomes reconstituted on hsp70 promoter DNA. In the presence of NURF, nucleosomes adopt one predominant position from an ensemble of possible locations within minutes. Movements occur in cis, with no transfer to competing DNA. Migrating intermediates trapped by fro III digestion reveal progressive nucleosome motion in increments of several base pairs. All four core histones are retained quantitatively during this process, indicating that the general integrity of the histone octamer is maintained. We suggest that NURF remodels nucleosomes by transiently decreasing the activation energy for short-range sliding of the histone octamer.
引用
收藏
页码：833 / 842
页数：10
相关论文
共 77 条
  • [21] ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor
    Ito, T
    Bulger, M
    Pazin, MJ
    Kobayashi, R
    Kadonaga, JT
    [J]. CELL, 1997, 90 (01) : 145 - 155
  • [22] ITO T, 1999, IN PRESS GENES DEV
  • [23] Eukaryotic transcription: An interlaced network of transcription factors and chromatin-modifying machines
    Kadonaga, JT
    [J]. CELL, 1998, 92 (03) : 307 - 313
  • [24] ACCURATE AND EFFICIENT RNA POLYMERASE-II TRANSCRIPTION WITH A SOLUBLE NUCLEAR FRACTION DERIVED FROM DROSOPHILA EMBRYOS
    KAMAKAKA, RT
    TYREE, CM
    KADONAGA, JT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (03) : 1024 - 1028
  • [25] STRUCTURE OF CHROMATIN
    KORNBERG, RD
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1977, 46 : 931 - 954
  • [26] NUCLEOSOME DISRUPTION AND ENHANCEMENT OF ACTIVATOR BINDING BY A HUMAN SW1/SNF COMPLEX
    KWON, H
    IMBALZANO, AN
    KHAVARI, PA
    KINGSTON, RE
    GREEN, MR
    [J]. NATURE, 1994, 370 (6489) : 477 - 481
  • [27] Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer
    Längst, G
    Bonte, EJ
    Corona, DFV
    Becker, PB
    [J]. CELL, 1999, 97 (07) : 843 - 852
  • [28] THE YEAST SNF2/SWI2-PROTEIN HAS DNA-STIMULATED ATPASE ACTIVITY REQUIRED FOR TRANSCRIPTIONAL ACTIVATION
    LAURENT, BC
    TREICH, I
    CARLSON, M
    [J]. GENES & DEVELOPMENT, 1993, 7 (04) : 583 - 591
  • [29] Requirement of RSF and FACT for transcription of chromatin templates in vitro
    LeRoy, G
    Orphanides, G
    Lane, WS
    Reinberg, D
    [J]. SCIENCE, 1998, 282 (5395) : 1900 - 1904
  • [30] RECONSTITUTION OF MONONUCLEOSOMES - CHARACTERIZATION OF DISTINCT PARTICLES THAT DIFFER IN THE POSITION OF THE HISTONE CORE
    LINXWEILER, W
    HORZ, W
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (24) : 9395 - 9413
12345678