Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa

被引:17
作者
Chen, Miao [1 ]
Wang, Ke [1 ]
Lin, Bin [1 ,2 ,3 ]
机构
[1] Univ Hong Kong, Li Ka Shing Fac Med, Dept Anat, Pokfulam, Hong Kong, Peoples R China
[2] Univ Hong Kong, Li Ka Shing Fac Med, Inst Eye, Pokfulam, Hong Kong, Peoples R China
[3] Univ Hong Kong, Li Ka Shing Fac Med, State Key Lab Brain & Cognit Sci, Pokfulam, Hong Kong, Peoples R China
关键词
RETINAL GANGLION-CELLS; HORIZONTAL CELLS; GLUTAMATE RECEPTORS; SPATIAL ORDER; GENE-THERAPY; RD/RD MOUSE; RAT RETINA; RD MICE; ORGANIZATION; MELANOPSIN;
D O I
10.1371/journal.pone.0044036
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Retinal photoreceptors die during retinal synaptogenesis in a portion of retinal degeneration. Whether cone bipolar cells establish regular retinal mosaics and mature morphologies, and resist degeneration are not completely understood. To explore these issues, we backcrossed a transgenic mouse expressing enhanced green fluorescent protein (EGFP) in one subset of cone bipolar cells (type 7) into rd1 mice, a classic mouse model of retinal degeneration, to examine the development and survival of cone bipolar cells in a background of retinal degeneration. Our data revealed that both the development and degeneration of cone bipolar cells are independent of the normal activity of cone photoreceptors. We found that type 7 cone bipolar cells achieved a uniform tiling of the retinal surface and developed normal dendritic and axonal arbors without the influence of cone photoreceptor innervation. On the other hand, degeneration of type 7 cone bipolar cells, contrary to our belief of central-to-peripheral progression, was spatially uniform across the retina independent of the spatiotemporal pattern of cone degeneration. The results have important implications for the design of more effective therapies to restore vision in retinal degeneration.
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页数:14
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