Evaluation of current rapid HIV test algorithms in Rakai, Uganda

被引：49

作者：

Galiwango, Ronald M. ^{[1
]}

Musoke, Richard ^{[1
]}

Lubyayi, Lawrence ^{[1
]}

Ssekubugu, Robert ^{[1
]}

Kalibbala, Sarah ^{[1
]}

Ssekweyama, Viola ^{[1
]}

Mirembe, Viola ^{[1
]}

Nakigozi, Gertrude ^{[1
]}

Reynolds, Steven J. ^{[1
,3
,5
]}

Serwadda, David ^{[1
,4
]}

Gray, Ronald H. ^{[2
]}

Kigozi, Godfrey ^{[1
]}

机构：

[1] Rakai Hlth Sci Program, Kalisizo, Uganda

[2] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA

[3] NIAID, Immunoregulat Lab, Div Intramural Res, NIH, Bethesda, MD 20892 USA

[4] Makerere Coll Hlth Sci, Sch Publ Hlth, Kampala, Uganda

[5] Johns Hopkins Univ, Sch Med, Baltimore, MD 21218 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 2013年 / 192卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

HIV; Rapids testing; Rakai-Uganda; Algorithms; FIELD;

D O I：

10.1016/j.jviromet.2013.04.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid HIV tests are a crucial component of HIV diagnosis in resource limited settings. In Uganda, the Ministry of Health allows both serial and parallel HIV rapid testing using Determine, Stat-Pak and Uni-Gold. In serial testing, a non-reactive result on Determine ends testing. The performance of serial and parallel algorithms with Determine and Stat-Pak test kits was assessed. A cross-sectional diagnostic test accuracy evaluation using three rapid HIV test kits as per the recommended parallel test algorithm was followed by EIA-WB testing with estimates of the performance of serial testing algorithm. In 2520 participants tested by parallel rapid algorithms, 0.6% had weakly reactive result. Parallel testing had 99.7% sensitivity and 99.8% specificity. If Stat-Pak was used as the first screening test for a serial algorithm, the sensitivity was 99.6% and specificity was 99.7%. However, if Determine was used as the screening test, sensitivity was 97.3% and specificity was 99.9%. Serial testing with Stat-Pak as the initial screening test performed as well as parallel testing, but Determine was a less sensitive screen. Serial testing could be cost saving. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：25 / 27

页数：3

共 13 条

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