Sub-inhibitory concentrations of heavy metals facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes in water environment

被引:322
作者
Zhang, Ye [1 ]
Gu, April Z. [2 ]
Cen, Tianyu [1 ]
Li, Xiangyang [1 ]
He, Miao [3 ]
Li, Dan [1 ]
Chen, Jianmin [1 ]
机构
[1] Fudan Univ, Shanghai Key Lab Atmospher Particle Pollut & Prev, Dept Environm Sci & Engn, Shanghai 200433, Peoples R China
[2] Cornell Univ, Sch Civil & Environm Engn, Ithaca, NY 14853 USA
[3] Tsinghua Univ, Sch Environm, Environm Simulat & Pollut Control ESPC State Key, Beijing 100084, Peoples R China
基金
美国国家科学基金会;
关键词
Sub-inhibitory concentrations; Heavy metals; Antibiotic resistance; Horizontal transfer; SOS response; COOPERATIVITY; COSELECTION; PROMOTES; BACTERIA; QUALITY; PROTEIN; IMPACT;
D O I
10.1016/j.envpol.2018.01.032
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Although widespread antibiotic resistance has been mostly attributed to the selective pressure generated by overuse and misuse of antibiotics, recent growing evidence suggests that chemicals other than antibiotics, such as certain metals, can also select and stimulate antibiotic resistance via both co-resistance and cross-resistance mechanisms. For instance, tetL, merE, and oprD genes are resistant to both antibiotics and metals. However, the potential de novo resistance induced by heavy metals at environmentally-relevant low concentrations (much below theminimum inhibitory concentrations [MICs], also referred as sub-inhibitory) has hardly been explored. This study investigated and revealed that heavy metals, namely Cu(II), Ag(I), Cr(VI), and Zn(II), at environmentally-relevant and sub-inhibitory concentrations, promoted conjugative transfer of antibiotic resistance genes (ARGs) between E. coli strains. The mechanisms of this phenomenon were further explored, which involved intracellular reactive oxygen species (ROS) formation, SOS response, increased cell membrane permeability, and altered expression of conjugation-relevant genes. These findings suggest that sub-inhibitory levels of heavy metals that widely present in various environments contribute to the resistance phenomena via facilitating horizontal transfer of ARGs. This study provides evidence from multiple aspects implicating the ecological effect of low levels of heavy metals on antibiotic resistance dissemination and highlights the urgency of strengthening efficacious policy and technology to control metal pollutants in the environments. (C) 2018 Elsevier Ltd. All rights reserved.
引用
收藏
页码:74 / 82
页数:9
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