Polyion Complex Vesicles for Photoinduced Intracellular Delivery of Amphiphilic Photosensitizer

被引:166
作者
Chen, Huabing [1 ,2 ,3 ]
Xiao, Ling [4 ]
Anraku, Yasutaka [3 ]
Mi, Peng [5 ]
Liu, Xueying [4 ]
Cabral, Horacio [5 ]
Inoue, Aki [3 ]
Nomoto, Takahiro [5 ]
Kishimura, Akihiro [3 ,6 ,7 ]
Nishiyama, Nobuhiro [8 ,9 ]
Kataoka, Kazunori [3 ,4 ,5 ,8 ]
机构
[1] Soochow Univ, Jiangsu Key Lab Translat Res & Therapy Neuropsych, Suzhou 215123, Peoples R China
[2] Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Peoples R China
[3] Univ Tokyo, Grad Sch Engn, Dept Mat Engn, Bunkyo Ku, Tokyo 1138656, Japan
[4] Univ Tokyo, Grad Sch Med, Ctr Dis Biol & Integrat Med, Bunkyo Ku, Tokyo 1130033, Japan
[5] Univ Tokyo, Grad Sch Engn, Dept Bioengn, Bunkyo Ku, Tokyo 1138656, Japan
[6] Kyushu Univ, Fac Engn, Ctr Mol Syst, Nishi Ku, Fukuoka 8190395, Japan
[7] Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
[8] Univ Tokyo, Ctr NanoBio Integrat CNBI, Bunkyo Ku, Tokyo 1138656, Japan
[9] Tokyo Inst Technol, Div Polymer Chem, Chem Resources Lab, Midori Ku, Yokohama, Kanagawa 2268503, Japan
基金
中国国家自然科学基金; 日本学术振兴会;
关键词
BLOCK-COPOLYMERS; PHOTOCHEMICAL INTERNALIZATION; PHOTODYNAMIC THERAPY; SUPRAMOLECULAR NANOCARRIERS; COLORIMETRIC ASSAY; NANOPARTICLES; DRUG; LYSOSOMES; EFFICACY; ENHANCE;
D O I
10.1021/ja406992w
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Polymer vesicles formed by a pair of oppositely charged poly(ethylene glycol) (PEG)-based block aniomer and homocatiomer, termed "PICsomes", have tunable size, and are characterized by unique semipermeable property due to the flexible and tunable hydrophilicity of polyion complex (PIC) membranes. The PICsomes can encapsulate a variety of molecules in an inner aqueous phase just by a simple vortex mixing of solution, expecting their utility as nanocontainers of substances with biomedical interests. Here, we report on a new functionality of the PICsomes: photoinduced release of photoactive agents for intracellular drug delivery. A potent photosensitizer, Al(III) phthalocyanine chloride disulfonic acid (AlPcS2a), was efficiently incorporated into the PICsomes (11%(w/w)), and its quick release was induced by photoirracliation possibly due to the photochemical damage of the PIG membranes. The combination of a high-resolution fluorescent confocal microscopy and a lysosome membrane-specific staining method revealed that such photoinduced release of AlPcS2a occurred even in the lysosomes of living cells after endocytic internalization. Simultaneously, the released AlPcS2a photochemically affected the integrity of the lysosomal membranes, leading to the translocation of AlPcS2a and PICsomes themselves to the cytoplasm. Consequently, the AlPcS2a-encapsulated PICsomes (AIPcS2a-PICsomes) exhibited appreciably stronger photocytotoxicity compared with free AlPcS2a alone. Thus, the AlPcS2a-PICsomes have promising feasibility for the photodynamic therapy or the photoinduced cytoplasmic delivery of therapeutic molecules.
引用
收藏
页码:157 / 163
页数:7
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