p-Hydroxybenzoate esters metabolism in MCF7 breast cancer cells

被引：30

作者：

Dagher, Zeina ^{[2
]}

Borgie, Mireille ^{[2
]}

Magdalou, Jacques ^{[3
]}

Chahine, Ramez ^{[2
]}

Greige-Gerges, Helene ^{[1
,2
]}

机构：

[1] Lebanese Univ, Fac Sci, Doctoral Sch Sci & Technol, Bioact Mol Res Grp,Sect 2, Jdaidet El Matn, Lebanon

[2] Lebanese Univ, Fac Sci 2, Dept Chem & Biochem, Jdaidet El Matn, Lebanon

[3] Univ Henri Poincare, UMR CNRS 7561, Nancy 1, France

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2012年 / 50卷 / 11期

关键词：

MCF7; cells; homogenates; Parabens; Cytotoxicity; Metabolism; BODY TOPICAL APPLICATION; HUMAN LIVER; HUMAN CARBOXYLESTERASE; SUBSTRATE-SPECIFICITY; ESTROGENIC ACTIVITY; GENE-EXPRESSION; PARABENS; HYDROLYSIS; EXPOSURE; HUMANS;

D O I：

10.1016/j.fct.2012.08.013

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Parabens are among the most frequently used preservatives to inhibit microbial growth and extend the shelf life of a range of consumer products. The objective of the present study was to gain insight into the metabolism of parabens in breast cancer cells (MCF7) since they have demonstrated estrogenic activity towards these cells and have been detected in breast cancer tissues. The toxicity of parabens to MCF7 cells was determined using MTT assays. Hydrolysis of methyl-, butyl and benzyl-paraben to p-hydroxybenzoic acid was analyzed in cultured MCF7 cells and in cellular homogenates. Glucuronidation and sulfoconjugation were studied in MCF7 homogenates, and parabens were analyzed by HPLC. Methyl-paraben was shown to be far less toxic than butyl and benzyl-paraben. Parabens were completely stable in MCF7 homogenates whereas p-nitrophenyl acetate, a substrate type, underwent hydrolysis. MCF7 cell homogenates did not express glucuronidation and sulfoconjugation activities toward parabens. The higher stability of parabens may explain their accumulation in breast cancer tissue as previously reported in the literature. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：4109 / 4114

页数：6

共 41 条

[1] Metabolism of Parabens (4-Hydroxybenzoic Acid Esters) by Hepatic Esterases and UDP-Glucuronosyltransferases in Man [J].

Abbas, Suzanne ;

Greige-Gerges, Helene ;

Karam, Nancy ;

Piet, Marie-Helene ;

Netter, Patrick ;

Magdalou, Jacques .

DRUG METABOLISM AND PHARMACOKINETICS, 2010, 25 (06) :568-577

[2] Final Amended Report on the Safety Assessment of Methylparaben, Ethylparaben, Propylparaben, Isopropylparaben, Butylparaben, Isobutylparaben, and Benzylparaben as used in Cosmetic Products [J].

Andersen, F. Alan .

INTERNATIONAL JOURNAL OF TOXICOLOGY, 2008, 27 :1-82

[3] Effects of skin metabolism on percutaneous penetration of lipophilic drugs [J].

Bando, H ;

Mohri, S ;

Yamashita, F ;

Takakura, Y ;

HAshida, M .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1997, 86 (06) :759-761

[4] Measurement of paraben concentrations in human breast tissue at serial locations across the breast from axilla to sternum [J].

Barr, L. ;

Metaxas, G. ;

Harbach, C. A. J. ;

Savoy, L. A. ;

Darbre, P. D. .

JOURNAL OF APPLIED TOXICOLOGY, 2012, 32 (03) :219-232

[5] Mammalian lignans and genistein decrease the activities of aromatase and 17β-hydroxy steroid dehydrogenase in MCF-7 cells [J].

Brooks, JD ;

Thompson, LU .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 94 (05) :461-467

[6] Biotransformation of genistein and bisphenol A in cell lines used for screening endocrine disruptors [J].

Bursztyka, J. ;

Perdu, E. ;

Pettersson, K. ;

Pongratz, I. ;

Fernandez-Cabrera, M. ;

Olea, N. ;

Debrauwer, L. ;

Zalko, D. ;

Cravedi, J. P. .

TOXICOLOGY IN VITRO, 2008, 22 (06) :1595-1604

[7] Oestrogenic activity of parabens in MCF7 human breast cancer cells [J].

Byford, JR ;

Shaw, LE ;

Drew, MGB ;

Pope, GS ;

Sauer, MJ ;

Darbre, PD .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2002, 80 (01) :49-60

[8] 2′-deoxy-N4-[2-(4-nitrophenyl)ethoxycarbonyl]-5-azacytidine:: A novel inhibitor of DNA methyltransferase that requires activation by human carboxylesterase [J].

Byun, Hyang-Min ;

Choi, Si Ho ;

Laird, Peter W. ;

Trinh, Binh ;

Siddiqui, Maqbool A. ;

Marquez, Victor E. ;

Yang, Allen S. .

CANCER LETTERS, 2008, 266 (02) :238-248

[9] Mitochondrial localization of ERα and ERβ in human MCF7 cells [J].

Chen, JQ ;

Delannoy, M ;

Cooke, C ;

Yager, JD .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2004, 286 (06) :E1011-E1022

[10] UDP-glucuronosyltransferase 1A6 overexpression in breast cancer cells resistant to methotrexate [J].

Cristina de Almagro, M. ;

Selga, Elisabet ;

Thibaut, Remi ;

Porte, Cinta ;

Noe, Veronique ;

Ciudad, Carlos J. .

BIOCHEMICAL PHARMACOLOGY, 2011, 81 (01) :60-70

← 1 2 3 4 5 →