Rare-Earth-Doped Calcium Carbonate Exposed to X-ray Irradiation to Induce Reactive Oxygen Species for Tumor Treatment

被引:18
作者
Yang, Chun-Chen [1 ]
Wang, Wei-Yun [1 ]
Lin, Feng-Huei [2 ,3 ]
Hou, Chun-Han [4 ]
机构
[1] Natl Taiwan Univ, Dept Mat Sci & Engn, Taipei 10617, Taiwan
[2] Natl Taiwan Univ, Inst Biomed Engn, Taipei 10617, Taiwan
[3] Natl Hlth Res Inst, Inst Biomed Engn & Nanomed, Miaoli 35053, Taiwan
[4] Natl Taiwan Univ, Dept Orthoped Surg, Taipei 10617, Taiwan
关键词
calcium carbonate; cerium; X-ray; alternative tumor treatment; PHOTODYNAMIC THERAPY PDT; IONIZING-RADIATION; CANCER; NANOPARTICLES; PHOTOSENSITIZER; DEGRADATION; MECHANISMS; DELIVERY;
D O I
10.3390/ijms20051148
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Conventional photodynamic therapy (PDT) is limited by its penetration depth due to the photosensitizer and light source. In this study, we developed X-ray induced photodynamic therapy that applied X-ray as the light source to activate Ce-doped CaCO3 (CaCO3:Ce) to generate an intracellular reactive oxygen species (ROS) for killing cancer cells. The A549 cell line was used as the in vitro and in vivo model to evaluate the efficacy of X-ray-induced CaCO3:Ce. The cell viability significantly decreased and cell cytotoxicity obviously increased with CaCO3:Ce exposure under X-ray irradiation, which is less harmful than radiotherapy in tumor treatment. CaCO3:Ce produced significant ROS under X-ray irradiation and promoted A549 cancer cell death. CaCO3:Ce can enhance the efficacy of X-ray induced PDT, and tumor growth was inhibited in vivo. The blood analysis and hematoxylin and eosin stain (H&E) stain fully supported the safety of the treatment. The mechanisms underlying ROS and CO2 generation by CaCO3:Ce activated by X-ray irradiation to induce cell toxicity, thereby inhibiting tumor growth, is discussed. These findings and advances are of great importance in providing a novel therapeutic approach as an alternative tumor treatment.
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页数:13
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