Arctigenin protects against ultraviolet-A-induced damage to stemness through inhibition of the NF-κB/MAPK pathway

被引：8

作者：

Park, See-Hyoung ^{[2
]}

Cho, Jae Youl ^{[1
]}

Oh, Sae Woong ^{[1
]}

Kang, Mingyeong ^{[1
]}

Lee, Seung Eun ^{[1
]}

Yoo, Ju Ah ^{[1
]}

Jung, Kwangseon ^{[4
]}

Lee, Jienny ^{[5
]}

Lee, Sang Yeol ^{[3
]}

Lee, Jongsung ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Dept Genet Engn, Coll Biotechnol & Bioengn, Suwon 16419, Gyunggi Do, South Korea

[2] Hongik Univ, Dept Bio & Chem Engn, Sejong City 30016, South Korea

[3] Gachon Univ, Dept Life Sci, San 65, Seongnam Si 13120, Gyunggi Do, South Korea

[4] Skincure Life Sci Inst, Seongnam City 13216, Gyunggi Do, South Korea

[5] Anim & Plant Quarantine Agcy, Viral Dis Res Div, 177 Hyeoksin 8 Ro, Gimcheon Si 39660, Gyeongsangbuk D, South Korea

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2018年 / 282卷

关键词：

Arctigenin; Human mesenchymal stem cells; Stemness; UVA damage; HIF; UP-REGULATION; CELLS; DIFFERENTIATION; ADIPOGENESIS; INFLAMMATION; HYPOXIA; LIGNAN;

D O I：

10.1016/j.cbi.2018.01.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG. In addition, the UVA-induced reduction in the mRNA expression level of hypoxia-inducible factor (HIF)-1 alpha was significantly recovered by arctigenin. The antagonizing effect of arctigenin on UVA irradiation was mediated by reduced PGE(2) production through the inhibition of MAPKs (p42/44 MAPK, p38 MAPK, and JNK) and NF-kappa B. Overall, these findings suggest that arctigenin can ameliorate the reduced stemness of hMSCs induced by UVA irradiation. The effects of arctigenin are mediated by PGE2-cAMP signaling-dependent upregulation of HIF-1 alpha. Therefore, arctigenin could be used as an antagonist to attenuate the effects of UVA irradiation.

引用

页码：63 / 68

页数：6

共 22 条

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